Metabolomics

Dataset Information

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Untargeted LC-MS metabolomics for the analysis of micro-scaled extracellular metabolites from hepatocytes

ABSTRACT: Metabolome analysis in micro physiological models is a challenge due to the low volume of cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method we have detected 57 metabolites of which 27 showed >2-fold shifts after 72-hours incubation. We demonstrate that micro-scale CCM samples can be used for modelling micro-physiological temporal dynamics in metabolite intensities.

INSTRUMENT(S): Liquid Chromatography MS - Positive (LC-MS (Positive))

PROVIDER: MTBLS1794 | MetaboLights | 2021-01-04

REPOSITORIES: MetaboLights

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Dataset's files

Source:
Action DRS
190701_S2_LC02MS02_CMtrial_032_ISmix5a.abf Other
190701_S2_LC02MS02_CMtrial_033_CM control 1_200 1.abf Other
190701_S2_LC02MS02_CMtrial_034_CM control 1_200 2.abf Other
190701_S2_LC02MS02_CMtrial_035_CM control 1_200 3.abf Other
190701_S2_LC02MS02_CMtrial_036_CM day1 1_200 1.abf Other
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Publications

Untargeted LC-MS Metabolomics for the Analysis of Micro-scaled Extracellular Metabolites from Hepatocytes.

Abdalkader Rodi R   Chaleckis Romanas R   Meister Isabel I   Zhang Pei P   Wheelock Craig E CE   Kamei Ken-Ichiro KI  

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 20201218 7

Metabolome analysis in micro physiological models is a challenge due to the low volume of the cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method we have detected 57 metabolites of which 27 showed >2-fold shifts after 72-hour incubation. We demonstrate that micro-scale CCM samples can be used for modelling micro-physiological temporal dynami  ...[more]

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