Metabolomics,Multiomics

Dataset Information

4

Global Metabonomic and Proteomic Analysis of Human Conjunctival Epithelial Cells (IOBA-NHC) in Response to Hyperosmotic Stress


ABSTRACT: “Dry eye” is a multi-factorial inflammatory disease affecting the ocular surface. Tear hyperosmolarity in dry eye contributes to inflammation and cell damage. Recent research efforts on dry eye have been directed towards biomarker discovery for diagnosis, response to treatment and disease mechanisms. This study employed a spontaneously immortalized normal human conjunctival cell line, IOBA-NHC as a model to investigate hyperosmotic stress-induced changes of metabolites and proteins. Global and targeted metabonomic analyses, as well as proteomic analysis were performed on IOBA-NHC cells incubated in serum-free media at 280 mOsm (control), 380 mOsm and 480 mOsm for 24 h. Twenty-one metabolites and seventy-six iTRAQ-identified proteins showed significant changes under at least one hyperosmotic stress treatment as compared to controls. SWATH-based proteomic analysis further confirmed the involvement of inflammatory pathways such as prostaglandin 2 synthesis in IOBA-NHC cells under hyperosmotic stress. This study is the first to identify glycerophosphocholine synthesis and O-linked β-N-acetylglucosamine glycosylation as key activated pathways in ocular surface cells under hyperosmotic stress. These findings extend the current knowledge in metabolite markers of dry eye and provide potential therapeutic targets for its treatment.

OTHER RELATED OMICS DATASETS IN: PXD002661

INSTRUMENT(S): TripleTOF 5600 (AB Sciex)

SUBMITTER: Liyan Chen 

PROVIDER: MTBLS214 | MetaboLights | 2015-08-31

REPOSITORIES: MetaboLights

altmetric image

Publications

Global Metabonomic and Proteomic Analysis of Human Conjunctival Epithelial Cells (IOBA-NHC) in Response to Hyperosmotic Stress.

Chen Liyan L   Li Jing J   Guo Tiannan T   Ghosh Sujoy S   Koh Siew Kwan SK   Tian Dechao D   Zhang Liang L   Jia Deyong D   Beuerman Roger W RW   Aebersold Ruedi R   Chan Eric Chun Yong EC   Zhou Lei L  

Journal of proteome research 20150819 9


"Dry eye" is a multifactorial inflammatory disease affecting the ocular surface. Tear hyperosmolarity in dry eye contributes to inflammation and cell damage. Recent research efforts on dry eye have been directed toward biomarker discovery for diagnosis, response to treatment, and disease mechanisms. This study employed a spontaneously immortalized normal human conjunctival cell line, IOBA-NHC, as a model to investigate hyperosmotic stress-induced changes of metabolites and proteins. Global and t  ...[more]

Similar Datasets

2015-08-17 | PXD002661 | Pride
2017-04-28 | MSV000081036 | GNPS
2022-02-07 | GSE195578 | GEO
2022-07-18 | GSE208297 | GEO
| E-GEOD-27593 | biostudies-arrayexpress
| E-GEOD-28941 | biostudies-arrayexpress
| E-GEOD-72662 | biostudies-arrayexpress
| E-GEOD-72663 | biostudies-arrayexpress
2016-09-01 | GSE72663 | GEO
2016-09-01 | GSE72662 | GEO