Alterations in fecal metagenomics and serum metabolomics indicate management strategies for patients with Budd-Chiari syndrome

ABSTRACT:

OBJECTIVES: We investigated the effects of gut microbiota and serum metabolic levels on the patients with Budd-Chiari syndrome (B-CS), and their guidance for clinical management strategies.

DESIGN: 214 B-CS subjects including untreated case (n=93) and treated case (n=121), were enrolled in this study. Gut microbiota and serum metabolome were analyzed using shotgun metagenomics and liquid chromatography mass spectrometry (LC-MS). 41 controls were included.

RESULTS: The gut microbiota in B-CS patients showed greater abundance of Campylobacter and lower Saccharomyces, Deinococcus and Thiomonas (P < 0.05). 13 differential metabolites such as taurocholate and (R)−3−Hydroxybutyric acid were identified in B-CS patients. The random forest (RF) models showed that serum metabolome could effectively identify B-CS from healthy controls and RF-metabolomics exhibited perfect discrimination (AUC = 100%, 95% CI: 100% – 100%), which was significantly higher than that achieved by RF-metagenomics (AUC =58.48%, 95% CI: 38.46% – 78.5%). The results of association analysis showed that Campylobacter concisus and taurocholate were significantly positively correlated in B-CS patients with clinical manifestations (P < 0.05). Moreover, Actinobacteria was significantly increased in untreated B-CS patients vs. treated B-CS patients (P < 0.05). Campylobacter and Veillonella were significantly increased in treated B-CS patients vs. health controls (P < 0.05). In addition, Fewer discrepant microbes were found between treated and untreated groups for the patients without clinical manifestation.

CONCLUSIONS: We identified major alterations in the gut microbiota and serum metabolome of B-CS patients. Alterations in fecal metagenomics and serum metabolomics guided management strategies for the patients with Budd-Chiari syndrome.

INSTRUMENT(S): Liquid Chromatography MS -

PROVIDER: MTBLS2319 | MetaboLights | 2021-10-20

REPOSITORIES: MetaboLights