Metabolomics

Dataset Information

0

A Multi-omics Longitudinal Study Reveals Alteration of the Leukocyte Activation Pathway in COVID-19 Patients.


ABSTRACT: Severe coronavirus disease 2019 (COVID-19) infection may lead to lung injury, multi-organ failure, and eventually death. Cytokine storm due to excess cytokine production has been associated with fatality in severe infections. However, the specific molecular signatures associated with the elevated immune response are yet to be elucidated. We performed a mass-spectrometry-based proteomic and metabolomic analysis of COVID-19 plasma samples collected at two time points. Using Orbitrap Fusion LC-MS/MS-based label-free proteomic analysis, we identified around 10 significant proteins, 32 significant peptides, and 5 metabolites that were dysregulated at the severe time points. Few of these proteins identified by quantitative proteomics were validated using the multiple reaction monitoring (MRM) assay. Integrated pathway analysis using distinct proteomic and metabolomic signatures revealed alterations in complement and coagulation cascade, platelet aggregation, myeloid leukocyte activation pathway, and arginine metabolism. Further, we highlight the role of leukocyte activation and arginine metabolism in COVID-19 pathogenesis and targeting these pathways for COVID-19 therapeutics.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase

SUBMITTER: Nirjhar Banerjee  

PROVIDER: MTBLS2469 | MetaboLights | 2022-01-18

REPOSITORIES: MetaboLights

Dataset's files

Source:
Action DRS
MTBLS2469 Other
Blank Other
02122020_Blank_P_1.raw Raw
02122020_Blank_P_2.raw Raw
02122020_Blank_P_3.raw Raw
Items per page:
1 - 5 of 191
altmetric image

Publications

A Multi-omics Longitudinal Study Reveals Alteration of the Leukocyte Activation Pathway in COVID-19 Patients.

Suvarna Kruthi K   Salkar Akanksha A   Palanivel Viswanthram V   Bankar Renuka R   Banerjee Nirjhar N   Gayathri J Pai Medha M   Srivastava Alisha A   Singh Avinash A   Khatri Harsh H   Agrawal Sachee S   Shrivastav Om O   Shastri Jayanthi J   Srivastava Sanjeeva S  

Journal of proteome research 20210811 10


Severe coronavirus disease 2019 (COVID-19) infection may lead to lung injury, multi-organ failure, and eventually death. Cytokine storm due to excess cytokine production has been associated with fatality in severe infections. However, the specific molecular signatures associated with the elevated immune response are yet to be elucidated. We performed a mass-spectrometry-based proteomic and metabolomic analysis of COVID-19 plasma samples collected at two time points. Using Orbitrap Fusion LC-MS/M  ...[more]

Similar Datasets

2020-01-01 | S-EPMC7693119 | BioStudies
2022-03-22 | GSE188847 | GEO
2020-01-01 | S-EPMC7488591 | BioStudies
2020-01-01 | S-EPMC7557250 | BioStudies
| phs002576 | dbGaP
2020-01-01 | S-EPMC7480444 | BioStudies
2021-01-01 | S-EPMC7721347 | BioStudies
2020-01-01 | S-EPMC7188184 | BioStudies
2020-01-01 | S-EPMC7641188 | BioStudies
2020-01-01 | S-EPMC7195532 | BioStudies