Dataset Information

Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian Piper betle L. Variants

ABSTRACT: The differences in pungency of 'sirih' imply the probable occurrence of several variants of Piper betle L. in Malaysia. However, the metabolite profiles underlying the pungency of the different variants remain a subject of further research. The differences in metabolite profiles of selected Malaysian P. betle variants were thus investigated; specifically, the leaf aqueous methanolic extracts and essential oils were analyzed via ¹H-NMR and GC-MS metabolomics, respectively. Principal component analysis (PCA) of the ¹H-NMR spectral data showed quantitative differences in the metabolite profiles of 'sirih melayu' and 'sirih india' and revealed an ambiguous group of samples with low acetic acid content, which was identified as Piper rubro-venosum hort. ex Rodigas based on DNA sequences of the internal transcribed spacer 2 (ITS2) region. The finding was supported by PCA of two GC-MS datasets of P. betle samples obtained from several states in Peninsular Malaysia, which displayed clustering of the samples into 'sirih melayu' and 'sirih india' groups. Higher abundance of chavicol acetate was consistently found to be characteristic of 'sirih melayu'. The present research has provided preliminary evidence supporting the notion of occurrence of two P. betle variants in Malaysia based on chemical profiles, which may be related to the different genders of P. betle.

INSTRUMENT(S): Nuclear Magnetic Resonance (NMR) -, Gas Chromatography MS - positive

PROVIDER: MTBLS2585 | MetaboLights | 2022-03-21

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	PB_sirihindia_30mg_1_presat.fid.zip	Other
	PB_sirihindia_30mg_2_presat.fid.zip	Other
	PB_sirihindia_30mg_3_presat.fid.zip	Other
	PB_sirihindia_30mg_4_presat.fid.zip	Other
	PB_sirihindia_30mg_5_presat.fid.zip	Other

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Publications

Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian Piper betle L. Variants.

Osman Muhamad Faris MF Lee Soo Yee SY Sarbini Shahrul Razid SR Mohd Faudzi Siti Munirah SM Khamis Shamsul S Zainudin Badrul Hisyam BH Shaari Khozirah K

Plants (Basel, Switzerland) 20211119 11

The differences in pungency of "sirih" imply the probable occurrence of several variants of Piper betle L. in Malaysia. However, the metabolite profiles underlying the pungency of the different variants remain a subject of further research. The differences in metabolite profiles of selected Malaysian P. betle variants were thus investigated; specifically, the leaf aqueous methanolic extracts and essential oils were analyzed via 1H-NMR and GC-MS metabolomics, respectively ...[more]

PMID: 34834873

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Project description:The androgen receptor (AR) plays a key role in progression to incurable androgen-ablation resistant prostate cancer (PCA). We have identified three novel AR splice variants lacking the ligand binding domain (designated as AR3, AR4 and AR5) in hormone insensitive PCA cells. AR3, one of the major splice variants expressed in human prostate tissues, is constitutively active and its transcriptional activity is not regulated by androgens or antiandrogens. Immunohistochemistry analysis on tissue microarrays containing 429 human prostate tissue samples shows that AR3 is significantly upregulated during PCA progression and AR3 expression level is correlated with the risk of tumor recurrence after radical prostatectomy. Overexpression of AR3 confers ablation-independent growth of PCA cells while specific knock-down of AR3 expression (without altering AR level) in hormone resistant PCA cells attenuates their growth under androgen-depleted conditions in both cell culture and xenograft models, suggesting an indispensable role of AR3 in ablation-independent growth of PCA cells. Furthermore, AR3 may play a distinct yet essential role in ablation-independent growth through regulating a unique set of genes including AKT1, which are not regulated by the prototype AR. Our data suggest that aberrant expression of AR splice variants may be a novel mechanism underlying ablation-independence during PCA progression and AR3 may serve as a prognostic marker to predict patient outcome in response to hormonal therapy. Given that these novel AR splice variants are not inhibited by currently available anti-androgen drugs, development of new drugs targeting these AR isoforms may potentially be effective for treatment of ablation-resistant PCA. Total RNA was extracted from CWR-R1 and 22Rv1 cells treated with shAR3-1, shARa and the scrambled shRNA control, respectively. Each of CWR-R1 and 22Rv1 cells treated with shAR3-1 was compared with the scrambled shRNA control. The same experiments were performed for the cells treated with shARa.

Dataset Information

Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian Piper betle L. Variants

Dataset's files

Publications

Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian <i>Piper betle</i> L. Variants.

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