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Multi-omics toxicity profiling of engineered nanomaterials

ABSTRACT:

This study aims to implement methods for toxicological profiling of engineered nanomaterials using toxicogenomics, proteomics, and metabolomics along with computational analyses. For all three omics layers the human cell lines A549 (lung epithelial cells) and THP1 (monocytes) were separately exposed to the nanomaterials TiO2 NM104, MWNCT NM401, and Ag NM300k. Proteomics and metabolomics samples have been performed as biological triplicates and were taken after 0, 6, 12, 24, and 48 hours. To assess ecotoxic effects C. elegans worms were grown in soil treated with NM300k. Ecotox samples were taken only at 0 and 24 hours. Integrating all three omics layers will enable the identification of (novel) ENM specific modes of action (MoA).

Data availability:

Proteomic data associated with this study are available in the PRIDE repository: accession number PXD002401.

OTHER RELATED OMICS DATASETS IN: PRJNA125229

INSTRUMENT(S): Exactive (Thermo Scientific)

SUBMITTER: Marc Rurik

PROVIDER: MTBLS277 | MetaboLights | 2016-12-05

REPOSITORIES: MetaboLights

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