Dataset Information

Lipid biomarkers for algal resistance to viral infection in the ocean

ABSTRACT:

Marine viruses play a key role in regulating phytoplankton populations, greatly affecting the biogeochemical cycling of major nutrients in the ocean. Resistance to viral infection has been reported for various phytoplankton species under laboratory conditions. Nevertheless, the occurrence of resistant cells in natural populations is underexplored due to the lack of sensitive tools to detect these rare phenotypes. Consequently, our current understanding of the ecological importance of resistance and its underlying mechanisms is limited. Here, we sought to identify lipid biomarkers for the resistance of the bloom-forming alga Emiliania huxleyi to its specific virus, E. huxleyi virus (EhV). By applying an untargeted lipidomics approach, we identified a group of glycosphingolipid (GSL) biomarkers that characterize resistant E. huxleyi strains and were thus termed resistance-specific GSLs (resGSLs). Further, we detected these lipid biomarkers in E. huxleyi isolates collected from induced E. huxleyi blooms and in samples collected during an open-ocean E. huxleyi bloom, indicating that resistant cells predominantly occur during the demise phase of the bloom. Last, we show that the GSL composition of E. huxleyi cultures that recover following infection and gain resistance to the virus resembles that of resistant strains. These findings highlight the metabolic plasticity and coevolution of the GSL biosynthetic pathway and underscore its central part in this host-virus arms race.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS3323 | MetaboLights | 2023-06-12

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	1217_P_30_03_2017_101.CDF	Other
	A100_Blank_P_20_05_2017_13001.CDF	Other
	A10_374_V_T4_P_20_05_2017_1501.CDF	Other
	A11_374_V_T4_P_20_05_2017_5501.CDF	Other
	A12_374_VT4_P_20_05_2017_9501.CDF	Other

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Publications

Lipid biomarkers for algal resistance to viral infection in the ocean.

Schleyer Guy G Kuhlisch Constanze C Ziv Carmit C Ben-Dor Shifra S Malitsky Sergey S Schatz Daniella D Vardi Assaf A

Proceedings of the National Academy of Sciences of the United States of America 20230626 27

PMID: 37364132

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