Dataset Information

Piriformospora indica stimulates root metabolism of Arabidopsis thaliana

ABSTRACT: Piriformospora indica is a root-colonizing fungus, which interacts with a variety of plants including Arabidopsis thaliana. This interaction has been considered as mutualistic, as it leads to an increased mycelia dry weight and increased shoot fresh weight, respectively. So far, only indolic glucosinolates and phytohormones have been identified as major key players. In a comprehensive non-targeted metabolite profiling study by means of UPLC/ESI-QTOFMS and GC/EI-QMS we analyzed Arabidopsis thaliana’s roots, root exudates, and leaves of inoculated and non-inoculated plants and identified further biomarkers. Among them, the concentration of nucleosides, dipeptides, oligolignols, and glucosinolate degradation products was affected in the exudates. In the root profiles, nearly all metabolite levels increased upon co-cultivation, namely those of carbohydrates, organic acids, amino acids, glucosinolates, oligolignols, and flavonoids. In the leaf profiles, we detected by far less significant changes. We only observed an increased concentration in organic acids, carbohydrates, ascorbate, glucosinolates and hydroxycinnamic acids, and a decreased concentration in nitrogen-rich amino acids in inoculated plants.

INSTRUMENT(S): micrOTOF-Q II ESI-Qq-TOF (Bruker), 5975C Series GC/MSD (Agilent)

SUBMITTER: Steffen Neumann

PROVIDER: MTBLS341 | MetaboLights | 2016-07-15

REPOSITORIES: MetaboLights

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Publications

Piriformospora indica Stimulates Root Metabolism of Arabidopsis thaliana.

Strehmel Nadine N Mönchgesang Susann S Herklotz Siska S Krüger Sylvia S Ziegler Jörg J Scheel Dierk D

International journal of molecular sciences 20160708 7

Piriformospora indica is a root-colonizing fungus, which interacts with a variety of plants including Arabidopsis thaliana. This interaction has been considered as mutualistic leading to growth promotion of the host. So far, only indolic glucosinolates and phytohormones have been identified as key players. In a comprehensive non-targeted metabolite profiling study, we analyzed Arabidopsis thaliana's roots, root exudates, and leaves of inoculated and non-inoculated plants by ultra performance liq ...[more]

PMID: 27399695

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Project description:Root exudates are composed of primary and secondary metabolites known to modulate the rhizosphere microbiota. Glucosinolates are defense compounds present in the Brassicaceae family capable of deterring pathogens, herbivores and biotic stressors in the phyllosphere. In addition, traces of glucosinolates and their hydrolyzed byproducts have been found in the soil, suggesting that these secondary metabolites could play a role in the modulation and establishment of the rhizosphere microbial community associated with this family. We used Arabidopsis thaliana mutant lines with disruptions in the indole glucosinolate pathway, liquid chromatography-tandem mass spectrometry (LC-MS/MS) and 16S rRNA amplicon sequencing to evaluate how disrupting this pathway affects the root exudate profile of Arabidopsis thaliana, and in turn, impacts the rhizosphere microbial community. Chemical analysis of the root exudates from the wild type Columbia (Col-0), a mutant plant line overexpressing the MYB transcription factor ATR1 (atr1D) which increases glucosinolate production, and the loss-of-function cyp79B2cyp79B3 double mutant line with low levels of glucosinolates confirmed that alterations to the indole glucosinolate biosynthetic pathway shifts the root exudate profile of the plant. We observed changes in the relative abundance of exuded metabolites. Moreover, 16S rRNA amplicon sequencing results provided evidence that the rhizobacterial communities associated with the plant lines used were directly impacted in diversity and community composition. This work provides further information on the involvement of secondary metabolites and their role in modulating the rhizobacterial community. Root metabolites dictate the presence of different bacterial species, including plant growth-promoting rhizobacteria. Our results suggest that alterations in the indole glucosinolate pathway cause disruptions beyond the endogenous levels of the plant, significantly changing the abundance and presence of different metabolites in the root exudates of the plants as well as the microbial rhizosphere community.

Dataset Information

Piriformospora indica stimulates root metabolism of Arabidopsis thaliana

Publications

Piriformospora indica Stimulates Root Metabolism of Arabidopsis thaliana.

Similar Datasets

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