Dataset Information
Impact of in vitro hormone treatments on the bibenzyl production of Radula complanata (Untargeted metabolomics)
Ontology highlight
ABSTRACT: Bibenzyls are a specialized metabolite class found throughout the plant kingdom. One of the most prolific producers of bibenzyls are liverworts, specifically plants of the Radula genera. These plants possess an incredible diversity of bibenzyls, prenylated bibenzyls and a few (bis)bibenzyls, several of which have medicinal properties, including perrottetinene, an analog of tetrahydrocannabinol from cannabis. To provide insight into the bibenzyls’ biosynthesis in planta, exogenous phytohormones were applied to in vitro grown Radula complanata and bibenzyl metabolite production was monitored with targeted and untargeted metabolomics. The targeted metabolomic analysis of six prenylated bibenzyls revealed that production of these metabolites was largely reduced when plants were treated with abscisic acid (AA), salicylic acid (SA), 1-naphthaleneacetic acid (NAA) or 6-benzylaminopurine (BAP). The reduction of these metabolites in the BAP and NAA treatment suggests that prenylated bibenzyl production is negatively correlated with vegetative plant growth. The reduction of bibenzyls at low AA and SA concentrations and mild increase at higher AA and SA concentrations suggest that their production is regulated by these stress hormones. In addition, six other bibenzyl metabolites were tentatively identified from the untargeted analysis. These results provide insight into the influence of phytohormones on the bioactive bibenzyl content of R. complanata. Untargeted metabolomics is reported in the current study MTBLS3563. Targeted metabolomics is reported in MTBLS4321.
INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase
SUBMITTER: Kristian Peters Kaitlyn Blatt-Janmaat Steffen Neumann
PROVIDER: MTBLS3563 | MetaboLights | 2024-01-10
REPOSITORIES: MetaboLights
Publications
Impact of in vitro phytohormone treatments on the metabolome of the leafy liverwort Radula complanata (L.) Dumort.
Metabolomics : Official journal of the Metabolomic Society 20230309 3
<h4>Introduction</h4>Liverworts are a group of non-vascular plants that possess unique metabolism not found in other plants. Many liverwort metabolites have interesting structural and biochemical characteristics, however the fluctuations of these metabolites in response to stressors is largely unknown.<h4>Objectives</h4>To investigate the metabolic stress-response of the leafy liverwort Radula complanata.<h4>Methods</h4>Five phytohormones were applied exogenously to in vitro cultured R. complana ...[more]
