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A multi-omics approach reveals functions of Secretory Carrier-Associated Membrane Proteins in wood formation of Populus trees

ABSTRACT: Secretory Carrier-Associated Membrane Proteins (SCAMPs) are highly conserved proteins that are involved in membrane trafficking. An integrated approach was taken to elucidate function of SCAMPs in wood formation of Populus trees. The metabolomics analyses were performed in woody tissues of transgenic Populus trees carrying an RNAi construct for Populus tremula x tremuloides SCAMP3 (PttSCAMP3; Potri.019G104000). The secondary xylem of the transgenic trees displayed increases in the amounts of polysaccharide derived sugars as well as lignin oligomers, indicating increased deposition of both carbohydrate and lignin polymers in the secondary cell walls. Somewhat surprisingly, the transgenic lines showed significantly increased amounts of suberic acid in the secondary xylem tissues. Populus SCAMP proteins were shown to influence accumulation of secondary cell wall components including polysaccharides, polyphenolics, lipids and suberin in the woody tissues of Populus tree stems.

OTHER RELATED OMICS DATASETS IN: PXD008266

INSTRUMENT(S): 6530 Q-TOF LC/MS (Agilent)

SUBMITTER: Ilka Abreu

PROVIDER: MTBLS478 | MetaboLights | 2018-01-08

REPOSITORIES: MetaboLights

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A multi-omics approach reveals function of Secretory Carrier-Associated Membrane Proteins in wood formation of Populus trees.

Obudulu Ogonna O Mähler Niklas N Skotare Tomas T Bygdell Joakim J Abreu Ilka N IN Ahnlund Maria M Latha Gandla Madhavi M Petterle Anna A Moritz Thomas T Hvidsten Torgeir R TR Jönsson Leif J LJ Wingsle Gunnar G Trygg Johan J Tuominen Hannele H

BMC genomics 20180103 1

<h4>Background</h4>Secretory Carrier-Associated Membrane Proteins (SCAMPs) are highly conserved 32-38 kDa proteins that are involved in membrane trafficking. A systems approach was taken to elucidate function of SCAMPs in wood formation of Populus trees. Phenotypic and multi-omics analyses were performed in woody tissues of transgenic Populus trees carrying an RNAi construct for Populus tremula x tremuloides SCAMP3 (PttSCAMP3; Potri.019G104000).<h4>Results</h4>The woody tissues of the transgenic ...[more]

PMID: 29298676

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Project description:rs04-03_myb - myb - Xylogenesis is a fundamental developmental process that is specific of vascular plants. It allows the formation of xylem, also called wood in trees, a complex tridimensional tissue composed of different cell types. This process occurs through the control of fundamental cell mechanisms like cell division and differentiation, secondary cell wall synthesis, lignin deposition and programmed cell death. Xylogenesis is controlled spatially and temporally by specific genetic programs that involve hundreds of genes. For instance, lignin biosynthetic genes, CAD and CCR, are specifically expressed during xylogenesis through MYB transcription factor binding sites, a process that seems to be common to all vascular plants. We have cloned two xylem specific MYB transcription factors, EgMYB1 et EgMYB2, in Eucalyptus. Interestingly, they are able to bind MYB consensus sequences of CAD and CCR promoters in vitro and to modulate CAD and CCR expression in vivo. When overexpressed in Arabidopsis or tobacco, they affect xylem structure by changing cell wall structure and quality. To follow expression changes of Arabidopsis genes in transgenic plants overexpressing Eg MYB1 and EgMYB2 should help us to find out which genes might be target of those transcription factors. This should help us to decipher the actual role of those two MYBs in xylogenesis, two new members of a large family of transcription factors in plants. - Xylogenesis is a fundamental developmental process that is specific of vascular plants. It allows the formation of xylem, also called wood in trees, a complex tridimensional tissue composed of different cell types. This process occurs through the control of fundamental cell mechanisms like cell division and differentiation, secondary cell wall synthesis, lignin deposition and programmed cell death. Xylogenesis is controlled spatially and temporally by specific genetic programs that involve hundreds of genes. For instance, lignin biosynthetic genes, CAD and CCR, are specifically expressed during xylogenesis through MYB transcription factor binding sites, a process that seems to be common to all vascular plants. We have cloned two xylem specific MYB transcription factors, EgMYB1 et EgMYB2, in Eucalyptus. Interestingly, they are able to bind MYB consensus sequences of CAD and CCR promoters in vitro and to modulate CAD and CCR expression in vivo. When overexpressed in Arabidopsis or tobacco, they affect xylem structure by changing cell wall structure and quality. To follow expression changes of Arabidopsis genes in transgenic plants overexpressing Eg MYB1 and EgMYB2 should help us to find out which genes might be target of those transcription factors. This should help us to decipher the actual role of those two MYBs in xylogenesis, two new members of a large family of transcription factors in plants. Keywords: gene knock in (transgenic)

Dataset Information

A multi-omics approach reveals functions of Secretory Carrier-Associated Membrane Proteins in wood formation of Populus trees

Publications

A multi-omics approach reveals function of Secretory Carrier-Associated Membrane Proteins in wood formation of​ ​​Populus​​ ​trees.

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A multi-omics approach reveals function of Secretory Carrier-Associated Membrane Proteins in wood formation of Populus trees.