Local and systemic metabolic alterations in brain, plasma, and liver of rats in response to aging and ischemic stroke, as detected by nuclear magnetic resonance (NMR) spectroscopy

ABSTRACT: Cerebral ischemia and aging induce local and systemic effects, and impact severity of stroke outcome. We performed comprehensive metabolic profiling to detect metabolites in brain, plasma, and liver as a function of aging and ischemia/reperfusion (IR). Ischemic stroke was induced by middle cerebral artery occlusion. Abundant metabolites were identified and quantified by 1H-NMR. Older rats aged 12 months showed significant changes in metabolic profiles in the brain, but only a few metabolites were changed in the liver and plasma as compared to younger rats aged 3 months. However, I/R injury at day 2 resulted in major changes in metabolites in liver and plasma of older rats. In younger rats, focal cerebral ischemia induced reperfusion-time dependent changes in metabolites in brain, plasma, and liver. Metabolic changes were seen in brain as early as day 2 of I/R, plasma showed significant changes at day 3 of reperfusion, and liver exhibited delayed response after day 3 that continued to remain at week 2 of reperfusion-time. Pathways involved in energy and amino acid metabolism, inflammation, and oxidative stress were altered as a result of aging and I/R. Thus, age, tissue, and I/R time affect changes in metabolites and may have implications in stroke outcome.

INSTRUMENT(S): Bruker

PROVIDER: MTBLS516 | MetaboLights | 2019-02-01

REPOSITORIES: MetaboLights