Metabolomics

Dataset Information

2

Bile acid is a significant host factor shaping the gut microbiome of diet induced obese mice (untargeted metabolome profile assay)


ABSTRACT:

BACKGROUND: Intestinal bacteria are known to regulate the bile acid (BA) homeostasis via intestinal biotransformation of BAs and stimulating the expression of fibroblast growth factor 19 through intestinal nuclear farnesoid X receptor (FXR). On the other hand, BAs directly regulate the gut microbiota with their strong antimicrobial activities. It remains unclear, however, how mammalian BAs cross-talk with gut microbiome and shape microbial composition in a dynamic and interactive way.

RESULTS: We quantitatively profiled small molecule metabolites derived from host-microbial co-metabolism in mice, demonstrating that Bas were the most significant factor correlated with microbial alterations among all types of endogenous metabolites. HFD intervention resulted in a rapid and significant increase in intestinal BA pool within 12 hrs followed by alteration in microbial composition at 24 hrs, providing supporting evidence that BAs are major dietary factors regulating gut microbiota. Feeding mice with BAs along with normal diet induced obese phenotype and obesity-associated gut microbial composition, similar to HFD fed mice. Finally, inhibition of hepatic BA biosynthesis under HFD conditions attenuated the HFD-induced gut microbiome alterations. Inhibition of BAs and direct suppression of micriobiota improved obese phenotypes.

CONCLUSIONS: Our study highlights a liver - BAs - gut microbiome metabolic axis that drives significant modifications of BA and microbiota compositions capable of triggering metabolic disorders, suggesting new therapeutic strategies targeting BA metabolism for metabolic diseases.


The targeted short chain fatty acid analysis assay for this study can be found in the MetaboLights study MTBLS546.

The targeted bile acid analysis assay for this study can be found in the MetaboLights study MTBLS547.

The targeted fatty acid analysis assay for this study can be found in the MetaboLights study MTBLS548.

INSTRUMENT(S): TOF Pegasus HT (LECO)

SUBMITTER: Xiaojiao Zheng 

PROVIDER: MTBLS545 | MetaboLights | 2017-11-23

REPOSITORIES: MetaboLights

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Publications

Bile acid is a significant host factor shaping the gut microbiome of diet-induced obese mice.

Zheng Xiaojiao X   Huang Fengjie F   Zhao Aihua A   Lei Sha S   Zhang Yunjing Y   Xie Guoxiang G   Chen Tianlu T   Qu Chun C   Rajani Cynthia C   Dong Bing B   Li Defa D   Jia Wei W  

BMC biology 20171214 1


<h4>Background</h4>Intestinal bacteria are known to regulate bile acid (BA) homeostasis via intestinal biotransformation of BAs and stimulation of the expression of fibroblast growth factor 19 through intestinal nuclear farnesoid X receptor (FXR). On the other hand, BAs directly regulate the gut microbiota with their strong antimicrobial activities. It remains unclear, however, how mammalian BAs cross-talk with gut microbiome and shape microbial composition in a dynamic and interactive way.<h4>R  ...[more]

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