Dataset Information
Targeted plasma metabolomics combined with machine learning for the diagnosis of acute SARS-CoV-2
Ontology highlight
ABSTRACT: The routine clinical diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is largely restricted to real-time reverse transcription polymerase chain reaction (RT-qPCR), and tests that detect SARS-CoV-2 nucleocapsid antigen. Given the diagnostic delay and suboptimal sensitivity associated with these respective methods, alternative diagnostic strategies are needed for acute infection. We studied the use of a clinically validated liquid chromatography triple quadrupole method (LC/MS-MS) for detection of amino acids from plasma specimens. We applied machine learning models to distinguish between SARS-CoV-2-positive and negative samples and analyzed amino acid feature importance. A total of 200 samples were tested, including 70 from individuals with COVID-19, and 130 from negative controls. The top performing model overall allowed discrimination between SARS-CoV-2-positive and negative control samples with an area under the receiver operating characteristic curve (AUC) of 0.96 (95% confidence interval [CI] 0.91, 1.00), overall sensitivity of 0.99 (95%CI 0.92, 1.00), and specificity of 0.92 (95%CI 0.85, 0.95). This approach holds potential as an alternative to existing methods for the rapid and accurate diagnosis of acute SARS-CoV-2 infection.
INSTRUMENT(S): 2D Gas Chromatography MS – Positive
PROVIDER: MTBLS6739 | MetaboLights | 2024-08-26
REPOSITORIES: MetaboLights
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| 54COVID.d.zip | Other | |||
| 55COVID.d.zip | Other | |||
| plasma_crp_01.d.zip | Other | |||
| plasma_crp_02.d.zip | Other | |||
| plasma_crp_03.d.zip | Other |
Items per page: 1 - 5 of 341 |
Publications
Targeted plasma metabolomics combined with machine learning for the diagnosis of severe acute respiratory syndrome virus type 2.
Frontiers in microbiology 20230329
<h4>Introduction</h4>The routine clinical diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is largely restricted to real-time reverse transcription quantitative PCR (RT-qPCR), and tests that detect SARS-CoV-2 nucleocapsid antigen. Given the diagnostic delay and suboptimal sensitivity associated with these respective methods, alternative diagnostic strategies are needed for acute infection.<h4>Methods</h4>We studied the use of a clinically validated liquid chromatography ...[more]
