Dataset Information

The Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartments

ABSTRACT:

Apicomplexan parasite growth and replication relies on nutrient acquisition from host cells, in which intracellular multiplication occurs, yet the mechanisms that underlie the nutrient salvage remain elusive. Numerous ultrastructural studies have documented a plasma membrane invagination with a dense neck, termed the micropore, on the surface of intracellular parasites. However, the function of this structure remains unknown. Here we validate the micropore as an essential organelle for endocytosis of nutrients from the host cell cytosol and Golgi in the model apicomplexan Toxoplasma gondii. Detailed analyses demonstrated that Kelch13 is localized at the dense neck of the organelle and functions as a protein hub at the micropore for endocytic uptake. Intriguingly, maximal activity of the micropore requires the ceramide de novo synthesis pathway in the parasite. Thus, this study provides insights into the machinery underlying acquisition of host cell-derived nutrients by apicomplexan parasites that are otherwise sequestered from host cell compartments.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Gas Chromatography MS - positive, Liquid Chromatography MS - positive - reverse phase

SUBMITTER: KaiHe He

PROVIDER: MTBLS7006 | MetaboLights | 2023-04-19

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	MTBLS7006	Other
	FILES	Other
	a_MTBLS7006_exp1_GC-MS_positive__metabolite_profiling.txt	Txt
	a_MTBLS7006_exp1_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS7006_exp1_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt

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Publications

The Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartments.

Wan Wenyan W Dong Hui H Lai De-Hua DH Yang Jiong J He Kai K Tang Xiaoyan X Liu Qun Q Hide Geoff G Zhu Xing-Quan XQ Sibley L David LD Lun Zhao-Rong ZR Long Shaojun S

Nature communications 20230222 1

PMID: 36813769

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