Spatial metabolomics of in situ, host-microbe interactions (MALDI imaging MS assay)
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ABSTRACT: Spatial metabolomics describes the location and chemistry of small molecules involved in metabolic phenotypes, defense molecules and chemical interactions in natural communities. Most current techniques are unable to spatially link the genotype and metabolic phenotype of microorganisms in situ at a scale relevant to microbial interactions. Here, we present a spatial metabolomics pipeline (metaFISH) that combines fluorescence in situ hybridization (FISH) microscopy and high-resolution atmospheric pressure mass spectrometry imaging (AP-MALDI-MSI) to image host-microbe symbioses and their metabolic interactions. metaFISH aligns and integrates metabolite and fluorescent images at the micrometer-scale for a spatial assignment of host and symbiont metabolites on the same tissue section. To illustrate the advantages of metaFISH, we mapped the spatial metabolome of a deep-sea mussel and its intracellular symbiotic bacteria at the scale of individual epithelial host cells. Our analytical pipeline revealed metabolic adaptations of the epithelial cells to the intracellular symbionts, a variation in metabolic phenotypes in one symbiont type, and novel symbiosis metabolites. metaFISH provides a culture-independent approach to link metabolic phenotypes to community members in situ - a powerful tool for microbiologists across fields.
MALDI imaging MS assay protocols and data are reported in the current study MTBLS744.
UPLC-MS/MS assay protocols and data associated to this study are reported in MTBLS746.
On-tissue MALDI imaging MS/MS assay protocols and data associated to this study are reported in MTBLS805.
MRMS assay protocols and data associated to this study are reported in MTBLS811.
INSTRUMENT(S): MS Imaging - Atmospheric Pressure Matrix Assisted Laser Desorption/Ionization orbitrap MS - Positive
SUBMITTER: Manuel Liebeke
PROVIDER: MTBLS744 | MetaboLights | 2019-11-15
REPOSITORIES: MetaboLights
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