Dataset Information

Does Storage Post Sampling Influence the Stability of Sebum when used for Mass Spectrometry Metabolomics Analysis?

ABSTRACT:

Sebum is a biofluid excreted by sebaceous glands in the skin. In recent years sebum has been shown to contain endogenous metabolites diagnostic of disease, with remarkable results for Parkinson’s Disease. Given that sebum sampling is facile and non-invasive, its potential for use in clinical biochemistry diagnostic assays should be explored including the parameters for standard operating procedures around collection, transport and storage. To this aim we have here investigated the reproducibility of mass spectrometry data from sebum in relation to both storage temperature and length of storage. Sebum samples were collected from volunteers and stored for up to 4 weeks at a range of temperatures: ambient (circa 17 °C), -20 °C and -80 °C. Established extraction protocols were employed and the samples were analysed by two chromatographic mass spectrometry techniques and data investigated using PCA, PLS-DA and ANOVA. We cannot discriminate samples as a function of storage temperature or time stored in unsupervised analysis using data acquired via TD-GC-MS and LC-IM-MS, although the sampling of volatiles was susceptible to batch effects. This study indicates that the requirements for storage and transport of sebum samples that may be used in clinical assays are far less stringent than for liquid samples and indicate that sebum is suitable for remote and at home sampling prior to analysis.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase, Gas Chromatography MS - positive

PROVIDER: MTBLS7946 | MetaboLights | 2025-07-15

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	GC_ID100_20C_timepoint2weeks.mzXML	Mzxml
	GC_ID100_20C_timepoint4weeks.mzXML	Mzxml
	GC_ID100_80C_timepoint2weeks.mzXML	Mzxml
	GC_ID100_80C_timepoint4weeks.mzXML	Mzxml
	GC_ID100_AMBIENT_timepoint2weeks.mzXML	Mzxml

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How storage post sampling influences the stability of sebum when used for mass spectrometry metabolomics analysis?

Walton-Doyle Caitlin C Sinclair Eleanor E Begum Humayra H Hollywood Katherine A KA Trivedi Drupad K DK Barran Perdita P

Scientific reports 20240917 1

PMID: 39289421

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