Untargeted metabolomics analysis of gingival tissue in patients with severe periodontitis

ABSTRACT: Periodontitis, as a chronic inflammatory condition, its initiating factor is the plaque biofilm. The inflammation starts in the gingival tissue, if left untreated, may progress to severe periodontitis and cause bone and tooth loss. The purpose of this study was to determine the potential metabolic biomarkers and therapeutic drugs in the gingival tissue of patients with periodontitis in order to improve osteogenic differentiation in patients with severe periodontitis. The gingival tissue samples of 20 healthy controls and 20 patients with severe periodontitis were analyzed using ultra-performance liquid chromatography-mass spectrometry and ultra-performance gas chromatography-mass spectrometry in this study. Variable important in projection (VIP) values obtained from the Orthogonal partial least squares discrimination analysis (OPLS-DA) model were applied to identify differential metabolites. A two-tailed Student's t-test was further used to verify whether the differential metabolites between groups were significant, and the differential metabolites with VIP values > 1.0 and P values < 0.05 were selected for screening. In total, 65 metabolites were enriched in 33 metabolic pathways, 40 metabolites were increased significantly, and 25 metabolites expressed downregulation significantly. In addition, it was found that patients with severe periodontitis have abnormalities in metabolic pathways such as glucose metabolism, purine metabolism, amino acid metabolism, and so on. Furthermore, based on a multidimensional analysis, 12 different metabolites may be the potential biomarkers of severe periodontitis. Moreover, osteogenesis differentiation characteristics were detected in the selected metabolites. The findings may provide a basis for the study of diagnostic biomarkers and therapeutic metabolites to promote osteogenesis differentiation in severe periodontitis.

INSTRUMENT(S): Gas Chromatography MS - positive, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS8357 | MetaboLights | 2026-09-08

REPOSITORIES: MetaboLights