Deep Characterisation of the sn-Isomer Lipidome Using High-Throughput Data-Independent Acquisition and Ozone-Induced Dissociation
Ontology highlight
ABSTRACT: We report a workflow that enables untargeted characterization of hundreds of sn-resolved glycerophospholipid isomers from biological extracts in less than 20 min. It reveals that sn-isomer populations are tightly regulated and significantly different between cell lines and enables identification of rare lipids containing ultra long chain monounsaturated fatty acyl chains at the sn-1 position. It provides an order of magnitude increases over the number of sn-isomer pairs previously reported and is coupled with an automated data analysis pipeline.
INSTRUMENT(S): Direct infusion MS - positive
PROVIDER: MTBLS8628 | MetaboLights | 2023-12-29
REPOSITORIES: MetaboLights
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