Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chronic exposure to tire rubber-derived contaminant 6PPD-quinone impairs sperm quality and induces the damage of reproductive capacity in male mice

ABSTRACT:

The study investigates the effects of 6PPDQ on male fertility in adult mice. The findings of this study are essential as the effects of low-dose environmental factors of 6PPDQ on adult male infertility are unclear, with the increasing in male infertility. The results suggest that 6PPDQ adversely affects sperm quality and male fertility possibly through affecting the proliferation of germ cells and sperm function. Future study is warranted to investigate the molecular mechanisms by which 6PPDQ affects germ cell and mature sperm functions. This study provides essential information for further study on the influence of 6PPDQ on sperm function and protective measures

INSTRUMENT(S): Liquid Chromatography MS - positive - hilic, Liquid Chromatography MS - negative - hilic

PROVIDER: MTBLS9432 | MetaboLights | 2025-07-14

REPOSITORIES: MetaboLights

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	NCM-T1-neg.raw	Raw
	NCM-T2-neg.raw	Raw
	NCM-T3-neg.raw	Raw
	NCM-T4-neg.raw	Raw
	NCM-T5-neg.raw	Raw

Items per page:

1 - 5 of 44

Similar Datasets

Sperm RNA: A window to idiopathic infertile couples

Project description:We examine how NGS sequencing of sperm can provide a window as to how particular perturbations of the sperm RNA profile from baseline may be indicative of male factor infertility, and may thus provide direction as to proper course of infertility treatment for couple. NGS RNA-seq of 72 sperm samples from male partner of couples undergoing fertility treatment

2015-07-09 | E-GEOD-65683 | biostudies-arrayexpress

Identification of a damaging variant rs35033974 associated with idiopathic male infertility through degradation of TEX101 protein and its transient interactome

Project description:Human TEX101 is a testis-specific cell membrane protein expressed exclusively in male germ cells and a validated biomarker of male infertility. TEX101 was suggested to function as a cell surface chaperone of numerous proteins involved in sperm migration and sperm-egg interaction. However, the precise functional roles of human TEX101 in spermatogenesis and fertilization are unknown. Here, we show that a common homozygous variant rs35033974 of TEX101 (G99V) with ~1% frequency in the general population may be associated with idiopathic male infertility. Spermatozoa from patients with homozygous rs35033974 exhibited near-complete degradation of variant G99V TEX101 protein and concomitant degradation of its interactome, as revealed by proteomic measurements. Substantially reduced levels of numerous testis-specific membrane proteins involved in sperm migration and sperm-oocyte fusion (including LY6K, IZUMO3 and ADAM29) were confirmed in spermatozoa of men with homozygous G99V variant. These men were previously diagnosed with idiopathic male infertility or oligospermia and failed the treatment by intrauterine insemination. Collectively, these data may facilitate diagnostics of idiopathic male infertility, provides a rational for selection of male infertility treatments and validate TEX101 and its interactome as targets to develop non-hormonal male contraceptives.

2024-11-02 | MSV000096278 | MassIVE

Proteomic changes associated with sperm fertilizing ability in meat type roosters

Project description:The molecular basis of male infertility is still not well understood. Furthermore, in vitro sperm quality tests are poorly correlated to in vivo fertility. In order to better understand sperm biology and determine potential fertility biomarkers, we used a quantitative bottom-up proteomic approach to identify proteins relative to sperm fertilizing ability in meat roosters, suffering from fertility reduction due to genetic selection side effects.

2021-08-17 | PXD022322 | Pride

Discontinuation of chronic delta-9-tetrahydrocannabinol diminishes impact on male fertility and the sperm epigenome in rhesus macaques

Project description:Cannabis is commonly used amongst reproductive age males. Our group and others have shown that chronic delta-9-tetrahydrocannabinol (THC) use adversely impacts male fertility, but less is known about the potential reversibility of these changes. Our study’s objective was to determine if THC discontinuation mitigates THC-associated changes in male reproductive health using a rhesus macaque (RM) model of daily THC edible consumption over a 280-day period (4 spermatogenic cycles). Testicular volume, serum male hormones, semen parameters, sperm DNA fragmentation, seminal fluid proteomics, and whole genome bisulfite sequencing (WGBS) of sperm DNA were assessed at pre-THC, moderate-THC, and heavy-THC dosing, and at 70 and 140 days after THC discontinuation. Chronic THC use resulted in significant testicular atrophy, increased gonadotropins, decreased serum sex steroids, and increased DNA fragmentation. THC discontinuation led to partial recovery of testicular volume, sex steroids and sperm DNA integrity. Seminal fluid proteome analysis revealed differential expression of proteins enriched for processes related to cellular secretion, immune response, and fibrinolysis. WGBS identified significant differential methylation in heavy-THC versus pre-THC sperm, with partial restoration of methylation after discontinuation of THC. Genes associated with differentially methylated regions (DMRs) were enriched for pathways involved in nervous system development and function. This is the first study demonstrating that chronic THC use in RMs adversely impacts male reproductive health, methylation of genes involved in development, and expression of proteins important for male fertility. THC discontinuation improves impacts to male fertility, including partial restoration of THC-associated sperm DMRs in genes important for development.

2023-05-18 | PXD039802 | Pride

Proteomic analysis reveals that topoisomerase 2A is associated with defective sperm head morphology

Project description:Male infertility is widespread and estimated to affect 1 in 20 men. Whilst in some cases the aetiology of the condition is well understood, for at least 50% of men, the underlying cause is yet to be classified. The majority of infertile men produce enough spermatozoa, however it is a combination of poor sperm motility, associated with a modified cell morphology that contribute to infertility. One of the major limitations of using morphology to inform male fertility status is that the evaluation is highly subjective, due to the fact that it has to be done by microscopic examination. As such, biomarkers of male infertility are needed to help establish a more consistent diagnosis. In the present study, we compared nuclear extracts from both high- and low-quality spermatozoa by LC-MS/MS based proteomic analysis. Our data shows that nuclear retention of proteins is a common facet amongst low quality male reproductive cells. In particular, we demonstrate that the presence of Topoisomerase 2A in sperm heads, is highly correlated to poor head morphology. Topoisomerase A is therefore a potential new biomarker for confirming male infertility in clinical practice.

2019-06-10 | MSV000083954 | MassIVE

Multiple reproductive tract-specific whey acidic protein four-disulfide core genes demonstrate functional requirement for sperm differentiation and function in mice

Project description:Our research defines the pivotal roles of the WFDC locus genes in male fertility, particularly in the regulation of sperm development and function. By employing CRISPR/Cas9 mediated gene editing strategy to study this gene family, we provided robust genetic evidence confirming their interconnected functions, which are crucial for sperm differentiation and maturation. Disruptions within these genes can result in infertility, highlighting their regulatory significance. These findings substantiate the potential of targeting EPPIN and related genes for male contraceptive development. Moreover, our study suggests that mutations in the WFDC locus could be instrumental in diagnosing and treating idiopathic male infertility, thereby presenting new therapeutic prospects for reproductive health.

2024-11-26 | PXD053290 | Pride

Gcn5-mediated histone acetylation governs nucleosome dynamics in spermiogenesis [ATAC-seq]

Project description:During mammalian spermatogenesis, male germ cells undergo dramatic reorganization of chromatin, whereby 90-99% of histones are evicted and replaced by protamines. This reorganization prominently features histone acetylation to loosen chromatin structure. Given the potential role of retained histones in fertility and early embryonic development, the genomic location of retained nucleosomes is of great interest. However, the ultimate position and mechanisms underlying nucleosome eviction/retention are poorly understood, including several studies utilizing MNase-seq methodologies, but reporting remarkably dissimilar locations. Here, we utilized ATAC-seq to determine the location of retained nucleosomes in mouse sperm and found enrichment at promoters, but also retention at inter- and intragenic regions, and repetitive elements. We further investigated nucleosome eviction/retention by generating pre-meiotic, germ cell specific, conditional knockout mice for the histone acetyltransferase Gcn5, a key histone acetyltransferase. Gcn5cKO germ cells exhibited abnormal chromatin dynamics during spermiogenesis, including diminished nucleosome eviction leading to increased histone retention in sperm. These mice exhibited severe reproductive phenotypes: abnormal sperm production, sperm morphology, and ultimately, male infertility. Our findings demonstrate Gcn5 mediated histone acetylation promotes chromatin accessibility and nucleosome eviction in spermiogenesis, and that loss of histone acetylation leads to defects that disrupt male fertility and potentially, early embryogenesis.

2019-11-07 | GSE134825 | GEO

Gcn5-mediated histone acetylation governs nucleosome dynamics in spermiogenesis [MNase-Seq]

2019-11-07 | GSE134560 | GEO

Gcn5-mediated histone acetylation governs nucleosome dynamics in spermiogenesis [RNA-Seq]

2019-11-07 | GSE134521 | GEO

High resolution human sperm proteome LC-MSMS

Project description:The aim of this study was to achieve a higher resolution of the human sperm proteome for better understanding of the molecular causes of male infertility. For this purpose, we examined the proteomic profiles of sperm from 76 men for proteins with deregulated abundances. Infertile men had abnormal semen parameters and were involuntarily childless. Men in the cohort with normal fertility had normozoospermia and had fathered children without medical assistance. Mass spectrometry analysis led to an in-depth reconstruction of the human sperm proteome. The corresponding genes were mainly involved in cellular motility, response to stimuli, adhesion, and reproduction. We also observed increasing numbers of at least three-fold differentially abundant sperm proteins from oligozoospermia and oligoasthenozoospermia to oligoasthenoteratozoospermia, compared with normozoospermia. Primarily, flagellar assembly and sperm motility, sperm-egg interaction, and male gametogenesis were affected. This suggests that sperm of infertile men carry signatures of impaired sperm production and are functionally impaired. Not least, we present new male infertility markers in addition to established ones.

2023-04-11 | PXD037531 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data