E.coli effects on growth and substrate uptake of green algae (part II - Reverse Phase)

ABSTRACT: The purpose of this project was to quantify the exchange of thiamine between bacteria and algae. We previously observed that the model bacteria, Escherichia coli, enhanced the growth and substrate uptake of the green algae, Auxenochlorella protothecoides. We hypothesized that this growth enhancement was due to the secretion of thiamine derivatives or degradation products by E. coli followed by uptake of these compounds by A. protothecoides. Targeted and untargeted LCMS revealed the presence of thiamine dervatives in E. coli cell extracts. These LCMS methods were also used to quantify thiamine derivatives and two degradation products, HMP and THZ, present in E. coli medium after cell removal. The LCMS results along with culture studies were employed to show that thiamine derivatives and degradation products were the primary mechanism of symbiosis between E. coli and A. protothecoides.

ORGANISM(S): Escherichia Coli E. Coli

TISSUE(S): Cells

SUBMITTER: Oliver Fiehn  

PROVIDER: ST000400 | MetabolomicsWorkbench | Fri May 13 00:00:00 BST 2016

REPOSITORIES: MetabolomicsWorkbench