Project description:Flash proteotyping is a methodology for ultra-fast identification of microorganisns by tandem mass spectrometry. Here, we obtained results on five reference strains and ten new bacterial isolates. The methodology is based on direct sample infusion into the mass spectromete and an original, highly sensitive procedure for data processing and taxonomic identification.
Project description:This work aimed to improve sensitivity of targeted detection by orbitrap mass spectrometers. Co-isolation of contaminant ions was identified as the major factor limiting sensitivity, and LOD of both PRM and accumulated precursor ion scanning (AIM) was improved by increased chromatographic resolution.