Metabolomics

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Untargeted LC-MS to compare blood collection tube and processing time – HILIC & C18


ABSTRACT: Blood was collected from three healthy volunteers in 3 blood collection tubes: serum separator tube SST (serum), EDTA (plasma), and P100 (plasma) and stored at 4 degrees for 0, 0.5, 1, 2, 4, and 24 hours prior to centrifugation. Compounds were extracted using liquid-liquid extraction to obtain a hydrophilic and a hydrophobic fraction and analyzed using liquid chromatography mass spectrometry. Differences among the blood collection tubes and sample processing time were evaluated (ANOVA with Bonferroni FWER ≤ 0.05 and ANOVA with Benjamini Hochberg FDR ≤ 0.1, respectively).

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Blood

SUBMITTER: Nichole Reisdorph  

PROVIDER: ST001099 | MetabolomicsWorkbench | Mon Feb 26 00:00:00 GMT 2018

REPOSITORIES: MetabolomicsWorkbench

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