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Metabolomic Markers of Methotrexate Response, In Vitro

ABSTRACT: Erythroblastoid cells (K562) maintained in logarithmic growth phase were treated with 1000 nM methotrexate or vehicle alone (i.e. D-PBS) under standard culture conditions for 24 hours. Cellular response to methotrexate was measured based on anti-proliferative activity by cell counting. Cells were washed, flash frozen in liquid nitrogen and submitted for metabolomics analysis to the NIH West Coast Metabolomics Center.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Cultured Cells

SUBMITTER: Ryan Funk

PROVIDER: ST001211 | MetabolomicsWorkbench | Wed Jul 03 00:00:00 BST 2019

REPOSITORIES: MetabolomicsWorkbench

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