Plasma lipidomic profiles after a low and high glycemic load dietary pattern in a randomized controlled cross over feeding study
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ABSTRACT:
ORGANISM(S): Homo Sapiens
TISSUE(S): Blood
PROVIDER: ST001490 | MetabolomicsWorkbench |
REPOSITORIES: MetabolomicsWorkbench
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Project description:BackgroundDietary patterns low in glycemic load are associated with reduced risk of cardiometabolic diseases. Improvements in serum lipid concentrations may play a role in these observed associations.ObjectiveWe investigated how dietary patterns differing in glycemic load affect clinical lipid panel measures and plasma lipidomics profiles.MethodsIn a crossover, controlled feeding study, 80 healthy participants (n = 40 men, n = 40 women), 18-45 y were randomized to receive low-glycemic load (LGL) or high glycemic load (HGL) diets for 28 days each with at least a 28-day washout period between controlled diets. Fasting plasma samples were collected at baseline and end of each diet period. Lipids on a clinical panel including total-, VLDL-, LDL-, and HDL-cholesterol and triglycerides were measured using an auto-analyzer. Lipidomics analysis using mass-spectrometry provided the concentrations of 863 species. Linear mixed models and lipid ontology enrichment analysis were implemented.ResultsLipids from the clinical panel were not significantly different between diets. Univariate analysis showed that 67 species on the lipidomics panel, predominantly in the triacylglycerol class, were higher after the LGL diet compared to the HGL (FDR < 0.05). Three species with FA 17:0 were lower after LGL diet with enrichment analysis (FDR < 0.05).ConclusionIn the context of controlled eucaloric diets with similar macronutrient distribution, these results suggest that there are relative shifts in lipid species, but the overall pool does not change. Further studies are needed to better understand in which compartment the different lipid species are transported in blood, and how these shifts are related to health outcomes. This trial was registered at clinicaltrials.gov as NCT00622661.
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Project description:Palmitic acid (PAM) can be provided in the diet or synthesized via de novo lipogenesis (DNL), primarily, from glucose. Preclinical work on the origin of brain PAM during development is scarce and contrasts results in adults. In this work, we use naturally occurring carbon isotope ratios (13C/12C; δ13C) to uncover the origin of brain PAM at postnatal days 0, 10, 21 and 35, and RNA sequencing to identify the pathways involved in maintaining brain PAM, at day 35, in mice fed diets with low, medium, and high PAM from birth. Here we show that DNL from dietary sugars maintains the majority of brain PAM during development and is augmented in mice fed low PAM. Importantly, the upregulation of hepatic DNL genes, in response to low PAM at day 35, demonstrates the presence of a compensatory mechanism to maintain total brain PAM pools compared to the liver; suggesting the importance of brain PAM regulation.
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Project description:Extracellular vesicles (EVs) are lipid bilayer nanovesicles secreted from almost all cells including cancer. Cancer-derived EVs contribute to cancer progression and malignancy via educating the surrounding normal cells. In breast cancer, epidemiological and experimental observations indicated that lipids are associated with cancer malignancy. However, lipid compositions of breast cancer EVs and their contributions to cancer progression are unexplored. In this study, we performed a widely targeted quantitative lipidomic analysis in cells and EVs derived from high- and low-metastatic triple-negative breast cancer cell lines, using supercritical fluid chromatography fast-scanning triple-quadrupole mass spectrometry. We demonstrated the differential lipid compositions between EVs and cells of their origin, and between high- and low-metastatic cell lines. Further, we demonstrated EVs from highly metastatic breast cancer accumulated unsaturated diacylglycerols (DGs) compared with EVs from lower-metastatic cells, without increasing the amount in cells. The EVs enriched with DGs could activate the protein kinase D signaling pathway in endothelial cells, which can lead to stimulated angiogenesis. Our results indicate that lipids are selectively loaded into breast cancer EVs to support tumor progression.
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Project description:Males and females from each SM/J and LG/J were placed on either a low-fat diet or high-fat diet immediately after weaning until 20 weeks of age. At 20 weeks, mice were sacrificed in late morning after a four-hour fast and liver tissue was collected from 4 males and 4 females from each strain and diet.
2014-01-30 | E-MTAB-2172 | biostudies-arrayexpress
Project description:This study aimed to examine the changes in lipid and metabolite profiles of ovariectomized (OVX) rats with diet-induced metabolic syndrome-associated osteoarthritis (MetOA) after supplementation with greenshell mussel (GSM) using an untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics approach. Ninety-six rats were fed with one of four diets: control, control supplemented with GSM + GSM, high fat/high sugar (HFHS), or high fat/high sugar enriched with GSM (HFHS + GSM). After 8 weeks on experimental diets, half of the rats in each group underwent OVX and the other half were sham operated. After being fed for an additional 28 weeks, blood samples were collected for the metabolomics analysis. Lipid and polar metabolites were extracted from plasma and analysed by LC-MS. We identified 29 lipid species from four lipid subclasses (phosphatidylcholine, lysophosphatidylcholine, diacylglycerol, and triacylglycerol) and a set of eight metabolites involved in amino acid metabolism (serine, threonine, lysine, valine, histidine, pipecolic acid, 3-methylcytidine, and cholic acid) as potential biomarkers for the effect of HFHS diet and GSM supplementation. GSM incorporation more specifically in the control diet generated significant alterations in the levels of several lipids and metabolites. Further studies are required to validate these findings that identify potential biomarkers to follow OA progression and to monitor the impact of GSM supplementation.
| S-EPMC8622240 | biostudies-literature
Project description:IntroductionSuboptimal feeding practices among caregivers contribute to nutritional-related health problems in children in low and middle-income countries, such as Tanzania. The continuum of the recommended feeding practices has been an utmost challenge among caregivers during the transition from health facilities to homes for improved under-five children's health.ObjectiveThis study assessed feeding practices, dietary diversities, and dietary adequacy among caregivers with under-five children in the Dodoma region, Tanzania.MethodsA community-based descriptive cross-sectional study in a quantitative research approach was adopted to study 289 randomly selected caregivers of under-five children in Dodoma region, Tanzania from March to August 2022. The World Health Organization Infant and Young Children Feeding Guidelines was the main data collection tool. Data were analyzed descriptively using a Statistical Package for Social Sciences computer software program version 25. Statistical limits were set at a 95% confidence interval and a 5% significance level.ResultsMean age was 26±6.47 of which 68.2% (n = 197) were females. Findings revealed 66.1% of caregivers had unsatisfactory feeding practices. It was observed that 67.8% of the caregivers fed their under-five children inadequately. Only 32.2% of them attained the minimum dietary diversity while 35.3% and 31.5% of caregivers demonstrated feeding practices < and > the recommended minimum Dietary diversity respectively. Moreover, 90.7% of caregivers fed their children group one food while 59.1% of them did not practice feeding the children snacks between meals. Feeding practice, dietary diversity, and dietary adequacy were significantly related to the caregiver's sex, education level, and socioeconomic status (p<0.05).ConclusionUnder-five children are at serious risk of nutritional problems as most caregivers in this study demonstrated unsatisfactory feeding practices on dietary diversities for dietary adequacy below the world health organization recommended Minimum Dietary diversity and Minimum Meal Frequency. Community-based nutritional education programs for caregivers need to be disseminated to the community level to address the problem accordingly.
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2010-12-15 | E-MEXP-2830 | biostudies-arrayexpress
Project description:The gut microbiota plays a critical role in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). Increased fructose consumption and inadequate copper intake are two critical risk factors in the development of NAFLD. To gain insight into the role of gut microbiota, fecal metabolites, obtained from rats exposed to different dietary levels of copper with and without high fructose intake for 4 weeks, were analyzed by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC × GC-TOF MS). In parallel, liver tissues were assessed by histology and triglyceride assay. Our data showed that high fructose feeding led to obvious hepatic steatosis in both marginal copper deficient rats and copper supplementation rats. Among the 38 metabolites detected with significant abundance alteration between groups, short chain fatty acids were markedly decreased with excessive fructose intake irrespective of copper levels. C15:0 and C17:0 long chain fatty acids, produced only by bacteria, were increased by either high copper level or high fructose intake. In addition, increased fecal urea and malic acid paralleled the increased hepatic fat accumulation. Collectively, GC × GC-TOF MS analysis of rat fecal samples revealed distinct fecal metabolome profiles associated with the dietary high fructose and copper level, with some metabolites possibly serving as potential noninvasive biomarkers of fructose induced-NAFLD.
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