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Isotope Tracing Analysis in Intestinal Organoids with fructose

ABSTRACT: We isolated and cultured Intestinal Organoids from Mouse. To trace the metabolism of fructose, intestinal organoids were cultured in unlabeled fructose-containing DMEM/F12 medium (10 mM D-fructose, 0 mM D-glucose, 2.5 mM L-glutamine) for 12 hr, and then switched into 13C labeled fructose-containing medium (10 mM U-[13C] D-fructose, Sigma-aldrich) for 6 hr.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Intestine

SUBMITTER: Yuanlong Hou

PROVIDER: ST001622 | MetabolomicsWorkbench | Wed Dec 09 00:00:00 GMT 2020

REPOSITORIES: MetabolomicsWorkbench

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