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Lipidomics dataset of Danio rerio optic nerve regeneration model

ABSTRACT: The right optic nerve of 1 year old female and male Danio rerio were crushed and collected three days after. Matching controls of uninjured eyes were also collected. The tissue was dissected from euthanized fish and “flash” frozen on dry ice in Eppendorf tubes. Due to the small size of the nerves, for each category (female crush, female control, male crush, male control) n=24 the samples were pooled. The brain from one male fish was also collected for control/calibration. Lipid extraction was done with the Bligh and Dyer [2] method, followed by untargeted liquid chromatography-mass spectrometry (LC MS-MS) lipid profiling using a Q-Exactive Orbitrap instrument coupled with Vanquish Horizon Binary UHPLC LC-MS system. The lipids were identified and quantified with LipidSearch 4.2.21 and the statistical analysis was conducted through Metaboanalyst 5.0.

ORGANISM(S): Danio Reiro Danio Rerio Zebrafish

TISSUE(S): Eye Tissue

DISEASE(S): Eye Disease

SUBMITTER: Sanjoy Bhattacharya

PROVIDER: ST001725 | MetabolomicsWorkbench | Fri Mar 12 00:00:00 GMT 2021

REPOSITORIES: MetabolomicsWorkbench

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