Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Untargeted primary metabolite profiling in Arabidopsis thaliana

ABSTRACT: The goal of this project is to compare the primary metabolite profile in different tissue types of the model plant Arabidopsis thaliana. Specifically, plants were grown hydroponically under the long-day (16hr light/day) condition at 21C. Tissue samples, including leaves, inflorescences, and roots were harvest 4 1/2 weeks post sowing. Untargeted primary metabolites profiling was carried out using GCTOF.

ORGANISM(S): Arabidopsis Thaliana

TISSUE(S): Leaves, Roots

SUBMITTER: Xuelin Wu

PROVIDER: ST002012 | MetabolomicsWorkbench | Thu Dec 09 00:00:00 GMT 2021

REPOSITORIES: MetabolomicsWorkbench

ACCESS DATA

Json Xml

Similar Datasets

Expression data from 29-day old Arabidopsis plants

Project description:Metal oxide engineered nanoparticles, which are widely used in diverse applications, are known to impact terrestrial plants. These nanoparticles have a potential to induce changes in plant tissue transcriptomes, and thereby the productivity. Here we looked at how the two commonly used nanoparticles, nano-titania (TiO2) and nano-ceria (CeO2) can impact the underlying mechanisms associated plant growth at genome level. We used microarrays to detail the global programme of gene expression underlying various physiological processes associated with growth and development, and identified distinct classes of up-regulated genes during this process. 29 day old Arabidopsis plants were selected for RNA extraction from roots and rosette leaves, followed by hybridization on Affymetrix microarrays. Arabidopsis plants were exposed twice during the germination stage (Days 0 and 4), and once during the primary rosette stage (Day 17), to 500mg/L concentration of nano-titania and nano-ceria, followed by extraction of RNA from 29-day old plant tissues (roots and rosette leaves) for Microarray analysis. 0.1mM KCl was used as control for nano-titania, whereas sterilized millipore water was used as control for nano-ceria.

2016-05-31 | E-GEOD-80461 | biostudies-arrayexpress

Seedling temperature responses in Arabidopsis RRS-7

Project description:Plants have the capacity to sense and respond to even small changes in ambient temperature. Numerous thermomorphogenic responses can be observed in Seedlings including elongation of the hypocotyl, petioles and the primary root while leaf blade areas are reduced. Main objective of the experiment was the assessment of tissue-specific transcriptome responses in Arabidopsis seedlings exposed to elevated ambient temperatures, 5 days old Arabidopsis seedlings grown at 20°C in LD were shifted for 3 or 24h to 28°C. Control plants were kept at 20°C. At time of harvest, cotyledons and roots were dissected and collected separately from the hypocotyl. To minimize circadian effects, plant material was grown in several successive batches and harvested only for a period of 30 min per timepoint on several consecutive days. Shifts and harvest was performed at midday to late afternoon (see ZT in overall design).

2019-02-12 | GSE126373 | GEO

Global transcriptomic analysis of Arabidopsis thaliana ecotype Columbia-0 subjected to magnesium deficiency

Project description:Magnesium (Mg) is essential for many biological processes in plant cells and its deficiency causes yield reduction in crop systems. Low Mg status reportedly impacts on photosynthesis, sucrose partitioning and biomass allocation. However, earlier responses to Mg deficiency are scarcely described. Generally, symptoms of nutrient deficiency appear in specific ages of leaves. Therefore, we hypothesised that transcriptional responses to Mg deficiency are different depending on the ages of leaves, and performed a global transcriptomic analysis in two types of leaves; source and sink leaves of the model plant species Arabidopsis thaliana to reveal the earlier responses to Mg deficiency. The global transcriptomic study revealed that short-term Mg deficiency triggers the expression of defence response genes in sink leaves. In roots, although short-term Mg deficiency enhanced the Mg2+ uptake from the environmnet, transcriptional levels of genes encoding putative Mg2+ transporters in roots were unchanged, suggesting non-transcriptional regulation of Mg2+ uptake in roots.

2020-04-29 | GSE140070 | GEO

Transcription profiling of Arabidopsis thaliana leaves as reaction to caterpillar (Trichoplusia ni) feeding and fungi (Laccaria bicolor) infection of the roots

Project description:Arabidopsis thaliana plants were grown from seeds. Roots of 9 day old plants were inoculated with L. bicolor. After 4 weeks, two T. ni larvae per plant fed for 24 h. Leaves were subsequently harvested, shock frozen in liquid nitrogen and prepared for RNA extraction

2020-06-01 | E-MTAB-8523 | biostudies-arrayexpress

The Arabidopsis spaceflight transcriptome: a comparison of whole plants to discrete root, hypocotyl and shoot responses to the orbital environment

Project description:transcriptome response of Arabidopsis cultivar Columbia and WS whole plants and plant tissue roots, hypocotyls and shoots to the spaceflight environment Arabidopsis thaliana was evaluated for its response to the spaceflight environment in three replicated experiments on the International Space Station. Two approaches were used; GFP reporter genes were used to collect gene expression data in real time within unique GFP imaging hardware, and plants were harvested on orbit to RNAlater for subsequent analyses of gene expression with using Affymetrix and SAGE transcriptome analyses. Three tissue types were examined (leaves, hypocotyls and roots) and compared to analyses conducted with whole plants. Transcriptome analyses with whole plants suggested that the spaceflight environment had little impact on the transcriptome of arabidopsis, however, closer examination of selected tissues revealed that there are a number of tissue-specific responses that arabidopsis employs to respond to this novel environment. This experiment was launched on STS-131 in 2010 and was supported by NASA grant NNX07AH27G - Transgenic Plant Biomonitors of Space Flight Exposure to R.J. Ferl and A-L. Paul.

2014-04-10 | GSE56659 | GEO

Expression data from Arabidopsis thaliana - Pseudomonas interaction

Project description:Some soil bacteria promote plant growth, including Pseudomonas species. With this approach we detected significant changes in Arabidopsis genes related to primary metabolism that were induced by the bacteria. Pseudomonas G62 was applied to roots of 18 day-old Arabidopsis seedlings and the transcriptional profile of whole seedlings after 6 hours of treatment was analyzed.

2010-10-07 | E-GEOD-24552 | biostudies-arrayexpress

Transcription profiling of Arabidopsis leaves and roots from fry1 mutants compared to ph1;4 (DCJ) controls

Project description:Transcript analysis in the fry1 arabidopsis mutant compared to the pht1;4 (DCJ) control in leaves and roots of the plant. The experiment was duplicated.

2010-04-01 | E-MEXP-2483 | biostudies-arrayexpress

Arabidopsis shade avoidance gene expression in adult plants

Project description:The goal of this study is to obtain genome-wide shade-responsive genes in adult plant. We grew plants under long-day condition with high R/FR ratio (simulated "sun" condition) for two weeks. Plants were treated with shade starting at ZT 4 or left in the sun. We prepared two replicates of each sample at 1 hour and 4 hours after sun and shade treatment and five plants were pooled for each replicate. Cotyledons, hypocotyls, and roots were removed from the samples, leaving leaves and apical tissue.

2015-03-18 | GSE66967 | GEO

Primary metabolome in roots and leaves of non-mycorrhizal and mycorrhizal seedlings by GC-QTOF-MS

Project description:This dataset compared mycorrhizal-associated alterations in the plant primary metabolome across multiple plant-mycorrhizal fungus combinations. Specifically, we inoculated a phylogenetically diverse set of temperate tree species with either arbuscular mycorrhizal or ectomycorrhizal fungi (the two major mycorrhizal lifestyles). We then assessed the primary metabolome in mycorrhizal and non-mycorrhizal roots and the corresponding leaves.

2022-10-30 | MSV000090616 | MassIVE

The root-colonizing endophyte Piriformospora indica supports nitrogen-starved Arabidopsis seedlings with N metabolites

Project description:The root-colonizing endophytic fungus Piriformospora indica promotes root and shoot growth of its host plants. We show that growth promotion of Arabidopsis leaves is abolished when the seedlings are grown on media with nitrogen (N) limitation. The fungus neither stimulated the total N content nor did it promote 15NO3- uptake from agar plates to the leaves of the host under N-sufficient or N-limiting conditions. However, when the roots were co-cultivated with 15N-labelled P. indica, more label can be detected in the leaves of N-starved host plants, but not of plants supplied with sufficient N. Amino acid and primary metabolite profiles, as well as expression analyses of N metabolite transporter genes suggest that the fungus alleviates the adaptation of its host to the N limitation condition. P. indica alters the expression of transporter genes which participate in relocation of NO3-, NH4+ and N metabolites from the roots to the leaves under N limitation. We propose that P. indica participates in the plant´s metabolomic adaptation to N limitation by delivering reduced N metabolites to the host, alleviating metabolic N starvation responses, and reprogramming the expression of N-metabolism related genes.

2023-10-19 | GSE239281 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data