Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Systemic impact of the expression of the mitochondrial alternative oxidase on Drosophila development

ABSTRACT: Despite the beneficial effects shown when the mitochondrial alternative oxidase AOX from Ciona intestinalis (Tunicata: Ascidiacea) is xenotopically expressed in mammalian and insect models, important detrimental outcomes have also been reported, raising concerns regarding its envisioned deployment as a therapy enzyme for human mitochondrial and related diseases. We have shown previously that AOX-expressing flies present a dramatic drop in pupal viability when the larvae are cultured on a low nutrient (LN) diet, indicating that AOX interferes with normal developmental metabolism. Here, we applied the metabolomics approach to gain insights into the molecular basis of the fatal developmental interaction between AOX expression and LN diet. We investigated the whole-body metabolome of AOX-expressing and control larvae cultured on SD and LN diets.

ORGANISM(S): Drosophila Melanogaster Fruit Fly

TISSUE(S): Larvae

SUBMITTER: Eric Dufour

PROVIDER: ST002069 | MetabolomicsWorkbench | Sat Jan 29 00:00:00 GMT 2022

REPOSITORIES: MetabolomicsWorkbench

ACCESS DATA

Json Xml

Similar Datasets

Drosophila CNS mitochondrial DNA dysfunction microarray

Project description:Mitochondrial DNA (mtDNA) encodes essential components of the respiratory chain and loss of mtDNA leads to mitochondrial dysfunction and neurodegeneration. Mitochondrial transcription factor A (TFAM) is an essential component of mtDNA replication and a regulator of mitochondrial copy number in cells. Studies have shown that TFAM knockdown leads to mitochondrial dysfunction and respiratory chain deficiencies. Using gene expression analysis, we aimed to investigate the effects of mtDNA dysfunction in the CNS at the molecular level. We used microarray analysis to investigate gene expression in cases of mitochondrial dysfunction in the CNS. RNA was purified from the late third instar larval CNS from control larvae, or larvae over-expressing mitochondrial transcription factor A (TFAM) in post-mitotic neurons using the neuron specific driver nsyb-Gal4. Three replicates are included for each condition.

2013-12-20 | E-GEOD-53509 | biostudies-arrayexpress

Precocious metamorphosis of silkworm by BmNPV infection in the latter half of the fifth instar larvae

Project description:The silkworm, Bombyx mori, is a complete metamorphosis insect and an economically important for silk production, the model to study insect physiology and biochemistry. Bombyx mori nucleopolyhedrovirus (BmNPV) is a principal pathogen of the silkworm and its host range is restricted to silkworm larvae, requiring interaction with silkworm larvae to accomplish virus replication. Prothoracic glands (PGs) are a model for synthetic ecdysone with regulating insect growth and development. In this study, day-4 fifth instar silkworm larvae were infected by BmNPV, the wandering silkworms appeared in the infected groups were 12 hours earlier than that in the control groups, and the ecdysone titer in infected larvae was significantly higher than that of the control larvae. Then, we used RNA sequencing (RNA-seq) to analyze silkworm PGs 48 h after BmNPV infection. The classifications of the 15 differential expression genes (DEGs) were mainly involved in the metabolic processes and pathways. The RT-qPCR results of the DEGs in the PGs of BmNPV-infected at 24, 48, and 72 h were generally consistent with the transcriptome data. The transcripts of BmTrypsin-1 and BmACSS3 were significantly increased from 24 to 72 h after BmNPV infection that they may be involved in the maturation process in the latter half of silkworm fifth instar larvae. These findings will help to address the interactions between BmNPV infection and host developmental response.

2021-03-02 | GSE167875 | GEO

Developmental transcriptome profiling of sexed Anopheles gambiae pre-adult stages using strand-specific RNA-seq.

Project description:Overall, the study aims at obtaining a comprehensive picture of the African malaria mosquito, Anopheles gambiae, transcriptome using high-coverage RNA-seq of sexed whole-insect samples collected at different developmental time points. This experiment focuses on male and female transcriptomes from 20 hour old embryos, 12 hour old 3rd instar larvae, 24 hour old 4th instar larvae and 10 hour old pupae sampled using a strand-specific RNA-seq approach.

2015-06-15 | E-MTAB-2880 | biostudies-arrayexpress

Expression profile of cuticular genes of silkworm, Bombyx mori

Project description:Insect cuticle plays essential roles in multiple physiological functions. During molting and metamorphosis, tremendous changes occur in silkworm cuticles. Silkworm is a model of Lepidoptera insects; however, little is known about the stage expression profiles of genes in cuticles of silkworm. In the present study, we selected 16 developmental stages, ranging from day 1 of the first instar larvae to day 8 of pupae, to perform microarray-based expression profiles. The data told us that various functions and physiological pathways were activated in the cuticle. Moreover, the expression profiles of cuticular protein genes, as the important components of cuticle, were investigated. The current study provides important insights for the functional study of insect cuticle and the regulation of insect cuticular protein genes.

2009-11-05 | GSE18878 | GEO

Developmental transcriptome of sexed Anopheles gambiae larvae and adult mosquitoes

Project description:Overall, the study aims at obtaining a comprehensive picture of the African malaria mosquito, Anopheles gambiae, transcriptome using high-coverage RNA-seq of sexed whole-insect samples collected at different developmental time points. This experiment focuses on transcriptomes of 1st, 2nd, 3rd and 4th instar male and female larvae, and 2 day old male and female adults.

2015-04-01 | E-MTAB-2527 | biostudies-arrayexpress

Expression profile of cuticular genes of silkworm, Bombyx mori

2009-11-04 | E-GEOD-18878 | biostudies-arrayexpress

Deep sequencing of small RNA libraries reveals dynamic expression patterns of miRNAs in multiple developmental stages of Bactrocera dorsalis

Project description:Four developmental stage small RNA libraries including Eggs, Larvae, Pupae and Adults were constructed, where a lot of known miRNAs were identified and many novel miRNAs were predicted. Comparison of their expression profiles in the four libraries suggested that Bactrocera dorsalis miRNAs are dynamically regulated throughout the life cycle.Further analysis of the expression and function of these miRNAs could increase our understanding of regulatory networks in the insect and lead to novel approaches to its control.

2014-12-31 | GSE50450 | GEO

UNIL-Spodoptera littoralis-Wild type and mutant TFs

Project description:There is currently little information on which trancription factors control the expression of defence genes in response to herbivory in Arabidopsis thaliana. We performed a whole-genome analysis of Arabidopsis plants after feeding by Spodoptera littoralis larvae. Wild-type and knockout mutants in different insect-inducible transcription factors were either untreated (control plants) or challenged for 8 days with S. littoralis larvae (insect challenged plants).

2013-02-11 | E-MTAB-1418 | biostudies-arrayexpress

Comparative transcription profiling of heritage maize lines

Project description:Three different maize lines were assayed for differential gene expression in mature leaf tissue. Leaves from the Oh43 maize line are more resistant to insect larvae damage than the original parents, lines Oh40B and W8. The goal of the project was to discover genes highly expressed in the Oh43 line that potentially contributes to insect resistance.

2012-08-16 | GSE40107 | GEO

Identification of (E)-β-farnesene impair the development and survival of Chilo suppressalis larval by disrupting insect hormone balance

Project description:In this study, an artificial feeding study containing (E)-β-farnesene (0.8g/kg) with C. suppressalis larvae was conducted to investigate its physical performance, and results revealed that the average 2nd instar duration in C. suppressalis significantly increased from 4.78 days to 6.31 days after Eβf treatment (P<0.001), and in 3rd instar C. larvae also significantly increased from 5.70 days to 8.00 days (P<0.001). There were no significant differences in 4th and 5th C. suppressalis larval between Eβf treatment and control(P>0.05). The mortality rates of 2nd C. suppressalis larvae were increased by 21.00-fold, compared with control(P<0.001), 3rd C. suppressalis larval increased by 6.39-fold(P<0.001). Comparative transcriptome analysis also showed that multiple DEGs involved in insect hormone biosynthesis pathway, the results of hormone assay in 3rd instar larvae showed the balance between juvenile hormones and ecdysteroids was disrupted. Eβf treatment increased juvenile hormones titers, but not the ecdysteroids. The results of qPCR were consistent with RNA-seq. Taken together, Eβf impairs the development and survival of C. suppressalis larvae by disrupting insect hormone balance. Moreover, the altered pathways were associated with carbohydrates, xenobiotics and cofactors and vitamins in C. suppressalis larvae. Altogether, our findings identify Eβf impair the development and survival of C. suppressalis larvae. Additionally, our data also revealed a potential mechanism that Eβf impaired the development and survival of C. suppressalis larval by disrupting insect hormone balance. These findings may contribute to the integrated control of C. suppressalis.

2022-05-04 | GSE179532 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data