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Xenopus tropicalis glycolysis and PPP inhibition

ABSTRACT: Stage 41 tadpoles were injected with 4 nmol of the glycolysis inhibitor, 2-deoxyglucose (2DG), or a tracer control to evaluate consequences of inhibition on metabolites 24 hours after treatment began. Similarly, tadpoles were incubated in DMSO control or 1 of 2 G6PD inhibitors (Dehydroepiandrosterone and g6pdi), to similarly assess the consequences of inhibiting the pentose phosphate pathway. We find that inhibition of glucose metabolism with 2DG results in a decrease in downstream glycolytic intermediates, confirming a reduction in activity of this pathway. G6PD inhibition was not as clear as changes were less consistent across treatments and downstream metabolites did not behave in a coordinated way, though impacts on other metabolic processes by these inhibitors may be fruitful for exploring how they perturb metabolism in the tadpoles.

ORGANISM(S): Western Clawed Frog Xenopus Tropicalis

TISSUE(S): Tail

SUBMITTER: Jeet Patel

PROVIDER: ST002270 | MetabolomicsWorkbench | Wed Aug 03 00:00:00 BST 2022

REPOSITORIES: MetabolomicsWorkbench

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