Dataset Information

Non-targeted screening of natural products from 288 fungal endophytes from Canadian fruit crops

ABSTRACT: Fungal endophytes often live in symbiotic relationships with various plant hosts, conferring positive effects to their host organism. These endophytes frequently produce a wide variety of secondary metabolites with bioactivities that are often responsible for the beneficial effects seen in the host, such as antifungal or anti-insectan activity. A large group of fungal endophytes isolated from Canadian fruit crops including blueberries, raspberries, cranberries, grapes, and pears, was analyzed using molecular networking by GNPS in an effort to simplify the process of examining a large dataset. Molecular networking increased the speed and efficiency of examining this dataset, permitting the dereplication of 60 known compounds and the discovery of seven putative novel compounds, which will be purified, characterized, and tested for bioactivity in future studies.

ORGANISM(S): Alpinaria Cf. Rhododendri Mollisia Cf. Nigrescens Plagiostoma Petiolophilum Epicoleosporium Ramularioides Phaeomoniellales Sp. Coniothyrium Ferrarisianum Discosia Rubi Alternaria Infectoria Cryptosporella Sp. Venturia Sp. Chaetopsis Sp. Hypoxylon Submonticulosum Thyronectria Cucurbitula Alternaria Arborescens Tympanidaceae Sp. Creosphaeria Sassafras Godronia Cassandrae Phyllostictina Vaccinii Xylaria Corniformis Unknown Lophiotrema Mucilaginosis Nemania Diffusa Godronia Cf. Cassandrae Barbatosphaeriaceae Sp. Diaporthe Phaseolorum Trichaptum Abietinum Botrytis Cinerea Proliferodiscus Sp. Didymella Pomorum Hypoderma Cf. Rubi Diaporthe Vaccinii Cytospora Ribis Kretzschmaria Hedjaroudei Anthostomella Sp. Nemania Sp. Paraphaeosphaeria Neglecta Fusarium Tricinctum Xylaria Sp. Alternaria/aspergillus/botrytis/anthostomella/claussenomyces/devriesia/godronia/leptodontidium/mycosphaerella/protoventuria/rhizosphaera/spondycladiopsis/tumularia/cladosporium/colletotrichum/coniochaeta/coniothyrium/creosphaeria/cryptosporella/cytospora/diaporthe/didymella/discostroma/epicoccum/fusarium/geastrumia/gloeophyllum/guignardia/herpotrichia/hypoxylon/kretzschmaria/lachnellula/lachnum/lophiostoma/microdochium/mollisia/nemania/nigrospora/paramycosphaerella/paraphaeosphaeria/penicillium/phaeoacremonium/phialocephala/phomopsis/phyllosticta/pilidium/plagiostoma/pleonectria/proliferodiscus/pseudoplectania/pyrenochaeta/pyrenopeziza/ramularia/rhytismataceae/sarea/seimatosporium/setomelanomma/sphaerulina/stemphylium/trichaptum/varicosporium/venturia/wettsteinina/xylaria Leptodontidium Sp. Stemphylium Globuliferum Aposphaeria Corallinolutea Didymella Sp. Cytospora Cf. Cedri Setomelanomma Holmii Pleosporales Sp. Phyllosticta Pyrolae Aureobasidium Cf. Subglaciale Phaeoacremonium Fraxinopennsylvanicum Colletotrichum Fioriniae Dothideaceae Sp. Microdochium Neoqueenslandicum Ramularia Sp. Phyllosticta Cf. Pyrolae Epicoccum Nigrum Lachnellula Calyciformis Cladosporium Cf. Cladosporioides Neodevriesiaceae Sp. Articulospora Atra Nigrospora Sphaerica Camaropella Sp. Sarea Difformis Alternaria Alternata Neocucurbitaria Sp. Paramycosphaerella Sp. Xylariaceae Sp. Aspergillus Amstelodami Hyaloscyphaceae Sp. Alternaria Cf. Alternata Mollisia Cf. Melaleuca Colletotrichum Cf. Truncatum Pyrenopeziza Sp. Geastrumia Polystigmatis Casaresia Cf. Sphagnorum Phialocephala Sp. Cladosporium Allicinum Seimatosporium Lichenicola Sphaerulina Vaccinii Rhytismataceae Sp. Nemania Serpens Coniochaeta Cf. Marina Penicillium Camponotum Lachnum Sp. Pilidium Concavum Gloeophyllum Sepiarium Nigrospora Sp. Pseudoplectania Nigrella Mollisia Sp. Xylaria Ellisii Neovaginatispora Sp.

TISSUE(S): Plant

SUBMITTER: Justin Renaud

PROVIDER: ST002482 | MetabolomicsWorkbench | Thu Jan 06 00:00:00 GMT 2022

REPOSITORIES: MetabolomicsWorkbench

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Project description:Worldwide, 20-25% of all harvested fruit and vegetables are lost annually in the field and throughout the postharvest supply handling chain due to spoilage by fungal pathogens. Most impactful postharvest pathogens exhibit necrotrophic lifestyles, resulting in rotting of the host tissues and complete loss of marketable commodities. Necrotrophic fungi can readily infect ripe fruit leading to the rapid establishment of disease symptoms. However, these pathogens generally fail to infect unripe fruit, or remain quiescent until host and environmental conditions stimulate a successful infection. Current research on necrotrophic infections of fruit was mainly focused on the host by characterizing genetic and physicochemical factors that inhibit or promote the disease. However, the pathogenicity and virulence strategies employed by necrotrophic pathogens in ripe and unripe fruit are mostly understudied. Here, we provide a first comparative transcriptomics study of fungal postharvest pathogens: Botrytis cinerea, Rhizopus stolonifer and Fusarium acuminatum, all of which display necrotrophic behavior when infecting fruit. We de novo assembled and annotated the transcriptomes of R. stolonifer, and F. acuminatum and performed a differential gene expression analysis comparing the three fungal transcriptomes during fruit infection with that of fungal in-vitro growth. Analysis of the differentially expressed genes for enrichment of functional annotations revealed shared strategies of the three fungi during infection of compatible (ripe fruit) and incompatible (unripe fruit) hosts. We furthermore selected candidate genes that are involved in these strategies to characterize their expression during infection of unripe and ripe-like fruit of the non-ripening (nor) tomato mutant, both of which are physiologically and biochemically similar to unripe wildtype fruit. By enabling a better understanding of fungal necrotrophic infection strategies, we move closer to generating accurate models of fruit diseases and development of early detection tools and effective management strategies.

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Non-targeted screening of natural products from 288 fungal endophytes from Canadian fruit crops

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