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Simultaneous targeting of PD-1 and IL2Rβγ with radiation therapy to inhibit pancreatic cancer growth and metastasis

ABSTRACT: Mice were orthotopically implanted with PK5L1940 cells then radiation therapy (8 Gy) administered 7 days post-implantation. PD1-IL2v and aCD25 dosed once per week beginning day 7 post-implantation. T-cells from spleen were obtained through sorting, cultured in the presence of 13C6 glucose for 6h, and glucose fluxes profiled via mass spectrometry-based metabolomics.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): T-cells

DISEASE(S): Cancer

SUBMITTER: Julie Haines

PROVIDER: ST002489 | MetabolomicsWorkbench | Tue Feb 21 00:00:00 GMT 2023

REPOSITORIES: MetabolomicsWorkbench

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Simultaneous targeting of PD-1 and IL2Rβγ with radiation therapy to inhibit pancreatic cancer growth and metastasis - T-cell tracing supernatants

Project description:C57BL/6 mice were orthotopically implanted with PK5L1940 cells. 8 Gy RT was administered 7 days post-implantation. PD1-IL2v and aCD25 dosed once per week beginning day 7 post-implantation. Spleens and lymph nodes were harvested 17 days post implantation and the sorted T-cells cultured in the presence of 13C6 glucose for 6h. This study contains longitudinal sampling of the cell culture supernatants.

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The immunocytokine PD1-IL2v overcomes resistance to tumor immunity, and synergizes with anti-PD-L1

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| PRJNA136427 | ENA

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Expression data from vehicle tumor associated macrophages (TAM) VS PLX3397 treated-TAM.

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