Hi-C sequencing

ABSTRACT: The genomic DNA from exponential phase cells was cross-linked and digested with 200 unit MboI enzyme (NEB) as previously described36. The cutting DNA ends were labelled with biotin-14-dCTP (TriLINK) followed by ligation. Purified DNA was sheared to a length of ~400 bp. Point ligation junctions were pulled down with Dynabeads MyOne Streptavidin C1 (Thermofisher). The Hi-C library for Illumina sequencing was prepped with the NEBNext Ultra II DNA library Prep Kit for Illumina (NEB) according to the manufacturers’ instructions. Fragments between 400 and 600 bp were paired-end sequenced on a HiSeq2000 platform (Illumina).

SUBMITTER: Xiaoli Xue 

PROVIDER: OEX00000016 | NODE | 2018-12-26

REPOSITORIES: NODE