Project description:The cell-specific role of lysosomal protease cathepsin D (CtsD) in liver fibrosis is not completely clear. This study reports that while CtsD is highly expressed in liver macrophages of cirrhotic patients, CtsD deletion in macrophages resulted in enhanced liver fibrosis. For this scope, a macrophage-CtsD knock-out mouse (CtsDMac) model was generated. Livers obtained from the mouse model and from its control were analyzed by shotgun label-free quantitative (LFQ) proteomics.
Project description:Liver macrophages play a major role in the control of infections in the liver and in the pathology associated with chronic liver diseases. It was recently shown that liver macrophages can have two different origins, however, the extent to which these populations are functionally distinct remains to be fully addressed. In this study, we compare the gene expression profile of liver resident and bone marrow derived liver macrophages in mouse, 6 weeks after total body irradiation and bone marrow transplantation.
Project description:Genes expression profile in liver macrophages isolated from wild type C57BL/6J mice fed either a high fat or normal diet for 18 weeks