Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNAseq of HMC3 cell treated with Akk-Caco2

ABSTRACT: To decipher the molecular mechanisms of Akkermansia muciniphia effecting on microglia in ASD through the "brain-gut" axis, we performed RNAseq using HMC3 cells treated with different Caco-2 cell secretion.

TISSUE(S): ['cell Lines From Brain Tissue']

SUBMITTER: huajun zheng

PROVIDER: OEX00014683 | NODE | 2021-10-13

REPOSITORIES: NODE

ACCESS DATA

Json Xml

Similar Datasets

RNAseq Iron-Treated iPSC-derived Microglia

Project description:Iron accumulation in microglia has been observed in AlzheimerÃ¢Â€Â™s disease and other neurodegenerative disorders and is thought to contribute to disease progression through various mechanisms including neuroinflammation. To study the interaction between iron accumulation and inflammation, we treated human induced pluripotent stem cell-derived microglia (iPSC-MG) with an increasing concentration of iron, in combination with inflammatory stimuli such as interferon gamma and amyloid ÃŽÂ², and performed RNA sequencing.

| EGAS00001006112 | EGA

RNAseq analysis of microglia isolated from treated brain slices or treated mice

Project description:RNAseq analysis of microglia isolated from treated brain slices or treated mice

| PRJNA647999 | ENA

RNAseq analysis of microglia isolated from treated brain slices or treated mice

Project description:Population based transcriptomic profiling was performed on microglia isolated from TNF or GMCSF treated brain slices or from LPS treated mice

2020-12-14 | GSE154853 | GEO

RNA-seq of control and TIA1-knockdown HMC3 cells

Project description:The study aims to utilize RNA-seq to characterize the transcriptomic alterations in TIA1-knockdown HMC3 cells compared to control cells, with the goal of identifying differentially expressed genes and elucidating the molecular pathways and biological processes regulated by TIA1, a key RNA-binding protein. By profiling gene expression changes following TIA1 depletion, the research seeks to uncover how this protein influences cellular functions in HMC3 cells, a model of human microglia critical for central nervous system immunity and homeostasis. Given the established role of TIA1 in mRNA metabolism and its links to neurodegenerative disorders like amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), this study holds relevance for understanding microglial gene regulatory mechanisms disrupted in disease contexts. Identifying TIA1-dependent transcriptomic signatures in HMC3 cells can provide insights into the functional consequences of TIA1 dysfunction in microglia, potentially revealing novel therapeutic targets or biomarkers for neurological diseases while advancing our understanding of RNA-mediated regulatory networks in immune cells of the brain.

2025-05-24 | GSE297158 | GEO

NRF2 binding in HMC3 human microglia

Project description:Alzheimer’s disease (AD) is characterized by amyloid-beta (Aβ) plaques and neurofibrillary tangles, accompanied by elevated oxidative stress and inflammation. Microglia, the resident macrophages in the brain, play a key protective role by clearing plaques and damaged neurons. NRF2 (Nuclear factor erythroid 2-related factor 2) is a master regulator of cytoprotection against oxidative stress, whose activation alleviates oxidative damage, neuroinflammation, and cognitive deficits in AD models. However, direct targets of NRF2 in microglia remain unclear. In this study, we demonstrate that NRF2 activation by CDDO-Im significantly suppresses inflammation in human microglial cells (HMC3) stimulated by IFN-γ or Aβ. Through integrative RNA-sequencing and ChIP-sequencing analysis of NRF2, we identified five representative NRF2 directly target genes involved in inflammation (e.g., IL6, CDK6) and another five in autophagy (e.g., TFE3, SQSTM1). Public single-cell transcriptomic data further underscored the critical role of microglia in NRF2-mediated autophagy regulation within AD brains. Our findings revealed new direct NRF2 target genes, highlighting dual role of NRF2 in suppressing inflammation and enhancing autophagy, thus providing novel insights for therapeutic interventions in AD.

2025-11-27 | GSE300540 | GEO

Brain Molecular Mechanisms in Rasmussen Encephalitis

Project description:Brain Molecular Mechanisms in Rasmussen Encephalitis

| PRJNA1365836 | ENA

RNA sequencing analysis of SW480 cells treated with Amuc_0904

Project description:We performed RNA sequencing analysis in SW480 cells treated with Amuc_0904 to study the molecular mechanisms through which Amuc_0904 regulates goblet cell signature.

2024-07-10 | GSE266220 | GEO

Deciphering molecular mechanisms of MEIS1-mediated prostate tumor suppression through RNAseq and ChIPseq - RNAseq data

Project description:Deciphering molecular mechanisms of MEIS1-mediated prostate tumor suppression through RNAseq and ChIPseq - RNAseq data

| PRJNA548764 | ENA

Gene expression profile of HCT116 and HT29 cells treated with D/L2HG

Project description:The oncometabolite, 2-hydroxyglutarate (2HG), has been directly implicated in carcinogenesis; however, the underlying molecular mechanisms remain poorly understood. To elucidate the molecular mechanisms through which 2HG contributes to cancer progression, we performed transcriptome analysis in 2HG-treated colorectal cancer HCT116 and HT29 cells.

2023-03-02 | GSE210167 | GEO