Project description:The aim of study is to evaluate whether salidroside (S), tyrosol (T) and hydroxytyrosol (H) which are dietary phenylethanoids of natural origins have an influence on reversing gut dysbiosis induced by metabolic syndrome (MetS) mice. C57 BL/6J female mice induced by high fructose diet were established. All mice were adapted to the environment for 7 days with normal diet and sterile drinking water (DW), and randomly divided into 6 groups. Mice in the ND group are fed with ND and treated with normal saline. Other groups were fed with high fructose (HFru) by administration of normal saline, salidroside (S), tyrosol (T) or hydroxytyrosol (H) for 12 weeks by intragastric gavage. Fresh feces from each mouse were collected one days before the end of the experiment and temporarily placed in sterile tubules, and then snap-frozen in liquid nitrogen. Total DNA from stool bacteria was extracted using QIAamp DNA stool mini kit from Qiagen (Germantown, MD, USA) according to the manufacturer’s instructions. Illumina HiSeq sequencing analysis of the DNA samples.16S rRNA gene sequence data further revealed that S, T and H could enhance the diversity of gut microbiota. In general, the abundance of Shigella, Acinetobacter, Lactobacillus, Staphylococcus and Sporosarcina had changed significantly. These findings suggest that S, T and H probably suppress lipid accumulation and to hepatoprotective effect and improve intestinal microflora disorders to attenuate metabolic syndromes.

2022-01-24 | GSE194127 | GEO

Vaginal epithelial gene expression in postmenopausal women suffering from vaginal dryness

Project description:The onset of menopause is accompanied by a dramatic increase in reported symptoms of vaginal dryness, soreness, irritation or itching, pain with intercourse and bleeding after intercourse. Collectively these affect 25-50% of women of post-menopausal age and significantly impact their quality of life. To examine how gene expression differs between these groups, surface vaginal epithelial cells were collected from postmenopausal women suffering from vaginal dryness and appropriate controls not suffering from dryness. Affymetrix GeneChip Human 1.0 ST microarrays were performed on RNA isolated from ten participants.

2011-11-12 | GSE26761 | GEO

Lettuce 16S rRNA gene amplicon sequence reads

Project description:Lettuce 16S rRNA gene amplicon sequence reads Raw sequence reads

| PRJNA486409 | ENA