Project description:Isolated brain capillaries are essential for analyzing the changes of protein expressions at the blood-brain barrier (BBB) under pathological conditions. The standard brain capillary isolation methods require the use of at least 5 mouse brains in order to obtain a sufficient amount and purity of brain capillaries. The purpose of this study was to establish a brain capillary isolation method from a single mouse brain for protein expression analysis.
Project description:The functions and protein expressions of the blood-brain barrier are changed during brain development after birth. The purpose of the present study was to develop a method to isolate brain capillaries from a single frozen neonatal mouse brain and elucidate the enrichment of brain capillaries by quantitative proteomic analysis. The brain capillary fraction was prepared by the optimized method from a single frozen mouse neonatal brain (postnatal day 7). The brain capillary fractions and brain lysates were digested by trypsin and analyzed by DIA.