Project description:Single-cell RNA sequencing was performed on bone marrow mononuclear of a patient with acute myeloid leukemia with erythroid differentiation of the blasts and on peripheral blood mononuclear cells of a patient with acute myeloid leukemia with megakaryocytic differentiation of the blasts. Raw data for this dataset can be found at the EGA under accession EGAS00001006819.
Project description:Sepsis is a serious systemic inflammatory reaction, which often leads to acute lung injury, and then affects lung function. This study aimed to explore the molecular mechanism of the interaction between il1b+ alveolar resident macrophages and pulmonary endothelial cells during sepsis induced lung injury using single-cell RNA sequencing technology.
Project description:A single hematopoietic stem cell can give rise to all blood cells with remarkable fidelity. Here, we define the chromatin accessibility and transcriptional landscape controlling this process in thirteen primary cell types that traverse the hematopoietic hierarchy. Exploiting the finding that enhancer landscapes better reflect cell identity than mRNA levels, we enable "enhancer cytometry" for accurate enumeration of pure cell types from complex populations. We further reveal the lineage ontogeny of genetic elements linked to diverse human diseases. In acute myeloid leukemia, chromatin accessibility reveals distinctive regulatory evolution in pre-leukemic HSCs (pHSCs), leukemia stem cells, and leukemic blasts. These leukemic cells demonstrate unique lineage infidelity, confirmed by single cell regulomes. We further show that pHSCs have a competitive advantage that is conferred by reduced chromatin accessibility at HOXA9 targets and is associated with adverse patient outcomes. Thus, regulome dynamics can provide diverse insights into human hematopoietic development and disease. Single-cell ATAC-seq of LMPPs, Monocytes, LSCs and Luekemic blast cells.
Project description:Development and evaluation of an autosampler for integrating nanoPOTS with LC-MS. Includes proteomic data from nanoPOTS-based sample preparation, including diluted peptides of Shewanella oneidensis MR-1, single cultured MCF10A cells, and single cells from three Acute Myeloid Leukemia (AML) cell lines (MOLM-14, K562, and CMK). Data was searched with MaxQuant.