Project description:Full clinical data for a cohort of 199 individuals with acute coronary syndrome.
Untargeted serum metabolomics using the Metabolon platform for individuals with ACS (n=156).
Serum metabolomics using the Nightingale Health (NMR) platform for individuals with ACS and controls (ACS, n=191; controls, n=961).
Project description:We discovered, through untargeted metabolomics, that creatine promotes ferroptosis in colorectal cancer. Our study identifies ERK2 as a creatine sensor that mediates active FSP1 and ferroptosis resistance, and highlights the potential of targeting tumor SLC6A8 for cancer treatment.
Project description:Multi-omics integration is highly effective for the holistic characterization of biological systems. However, current bioinformatics tools face limitations when incorporating metabolomics data, particularly for handling untargeted annotation, and when providing alternatives to predefined knowledge bases. Advanced algorithms required for multi-omics integration typically demand programming skills and statistical background, restricting their use to specialized users. Here, we introduce TurbOmics, a user-friendly web-based platform that enables researchers with diverse backgrounds to analyze metabolomics, proteomics, and transcriptomics data using an integrative workflow that includes advanced algorithms for multi-omics integration, while addressing key challenges associated with metabolomics data. The platform is available at https://proteomics.cnic.es/TurboPutative/TurbOmicsApp.html.
Project description:Human iPSCs and NSCs were engineered by AAVS1 and/or C13 safe-harbor TALENs which mediated targeted integration of various reporter genes at single or dual safe-harbor loci. Multiple clones of targeted human iPSCs were used to compare with parental untargeted NCRM5 iPSCs. Polyclonal targeted human NSCs were used to compare with their parental untargeted NCRM1NSCs or H9NSCs. Total RNA obtained from targeted human iPSCs or NSCs compared to untargeted control iPSCs or NSCs.