Project description:In order to obtain a global view on the transcriptional changes induced by the sex-inducing pheromone released by Seminavis robusta mating type plus cells (SIP+), an RNA-seq experiment was conducted. Therefore, purified SIP+ from RP-UPLC/MS was added to MT- cultures, 15 min before light onset after a prolonged dark period. MT- cultures without extract addition constituted the control conditions. Samples were taken after 15 min, 1 h and 3 h of illumination. An additional, non-treated MT- culture was harvested before illumination.
Project description:Purpose: The purpose of this study is to define the mechanism of antifungal action of the vanillin derivative, o-vanillin, against Cryptococcus neoformans Methods: mRNA profiles of C. neoformasn cells cultured with or without o-vanillin were generated by RNA-Seq, using Illumina GAIIx. The sequence reads that passed quality filters were mapped to reference genome and the normalized RPKM values were calculated by CLC Genomics Workbench. Results: o-vanillin significantly altered global transcript profiles, induces oxidative stress, and interferes mitochondrial functions in C. neoformans. Conclusions: o-vanillin can be considered as the effective antifungal drug candidate. mRNA profiles of the cells grown in the presence or absence of o-vanillin were generated by RNA-Seq using Illumina GAIIx.