Project description:Local invasion is a critical early step in metastatic cancer. This study investigated the invasion mechanisms of primary (IGR39) and metastatic (IGR37) melanoma cells at the single-cell level using single-probe single-cell mass spectrometry (SCMS). We detected 166 of 228 metabolites in IGR39 and 168 of 172 in IGR37.
Project description:Single-cell RNA-seq analysis of primary human bone marrow immune populations isolated from B-ALL patient samples (matched diagnosis, remission and relapse), and healthy bone marrow donor samples.
Project description:Single-cell RNA-seq analysis of primary human bone marrow immune populations isolated from B-ALL patient samples (matched diagnosis, remission and relapse), and healthy bone marrow donor samples.
Project description:This dataset profiles the single-cell transcriptional response of human bone marrow stromal cells (BMSCs) following treatment with exosomes. Primary BMSCs were isolated from human bone marrow aspirates and cultured under standard stromal cell conditions. Cells were treated for 48 hours with either pan-cancer–derived exosomes or control exosomes, then washed and processed for single-cell RNA sequencing.
Project description:We combined the Single-probe single cell MS(SCMS) experimental technique with a bioinformatics software package, SinCHet-MS (Single Cell Heterogeneity for Mass Spectrometry), to characterize changes of tumor heterogeneity, quantify cell subpopulations, and prioritize the metabolite biomarkers of each subpopulation.