Project description:By employing a global gene expression profiling, we performed analysis of the molecular pathways which are deregulated in prostate cancer cell line DU145 grown under tumorsphere forming condition and as standard attached monolayer culture. Both oriignal DU145 and its radioresistant derivative DU145-RR were analyzed
Project description:Prostate cancer stem-like cells were derived from DU145 cells as suspension spheres. DU145 cells were transduced with a CNTN1 over expressing retroviral vector and a control empty vector. DU145 spheres were transduced using a retroviral-based shRNA vector against CNTN1 and a scrambled control shRNA viral vector. Both cell lines were cultured over a couple of passages before RNA was collected.
Project description:Glioblastoma (GBM) derived sphere lines and adherent cell lines are an important tool for research in basic and translational neuro-oncology. Documentation of their genetic identity has become a requirement for scientific journals and grant applications to exclude cross-contamination and misidentification that lead to misinterpretation of results. Here, we report expression data for 26 samples including 4 GBM derived sphere lines (4 x 3 replicates), 2 GBM derived sphere lines passaged through intracranial transplantation (2x 1), 2 adherent GBM derived cell lines (2 + 2 x 3 replicates), 4 corresponding glioblastoma tumors and 2 non-tumor brain tissues.
Project description:We performed RNA-seq analysis of human prostate cancer cells and sphere-derived cancer stem cells (PCSCs) to analyze the differentially expressed genes between PCSCs and differentiated cells, and find the potential key factors in PCSCs