Project description:A library of hairpins was generated using TInDel Assembly to be tested for RNA thermometer activity. This method provides variation in sequence, length, and topology at the RNA level. The library was generated in vitro and cloned into an expression vector for screening. The library was amplified via PCR from the vector for submission for NGS.
Project description:CAMPARI2 CRISPR screening for SOCE modulators of ER stress. PC cells were sorted and sequenced for CRISPR whole KO library (De brie). Unsorted, SOrte din LOW PC and LOW PC Tunica treated fro 4hours were analysed.
Project description:AMO-1 cells were transduced with Brunello CRISPR screening library at MOI 0.3, and culture in the presnece of ceritinib. DNA was isolated and one step PCR was perfomed and gRNAs conferring to resistance or sensitivity was analysed using MAGECK-VISPR.
Project description:We developed a Ligation-based Library vs Library high-throughput Yeast Two-Hybrid (LLL-Y2H) screening system to explore protein interactions.