Genomics,Multiomics

Dataset Information

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Specific point mutation of base editor 3 eliminates off-target single nucleotide mutation


ABSTRACT: Specific point mutation of base editor 3 eliminates off-target single nucleotide mutation

TISSUE(S):

SUBMITTER: Hui,Yang 

PROVIDER: OEX003345 | NODE |

REPOSITORIES: NODE

Dataset's files

Source:
Action DRS
1588140448676.bam Bam
1588140474370.bam Bam
1588140479289.bam Bam
1588140508890.bam Bam
1588140883000.bam Bam
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Publications

A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects.

Zuo Erwei E   Sun Yidi Y   Yuan Tanglong T   He Bingbing B   Zhou Changyang C   Ying Wenqin W   Liu Jing J   Wei Wu W   Zeng Rong R   Li Yixue Y   Yang Hui H  

Nature methods 20200518 6


Cytosine base editors (CBEs) offer a powerful tool for correcting point mutations, yet their DNA and RNA off-target activities have caused concerns in biomedical applications. We describe screens of 23 rationally engineered CBE variants, which reveal mutation residues in the predicted DNA-binding site can dramatically decrease the Cas9-independent off-target effects. Furthermore, we obtained a CBE variant-YE1-BE3-FNLS-that retains high on-target editing efficiency while causing extremely low off  ...[more]

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