Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

1 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Function of miR-200c/141 in mouse mammary gland

ABSTRACT: To investigate the changes of gene expression in mouse mammary gland after knockout of miR-200c/141 in mammary luminal cells. Luminal cells from CKO (K8-CreERT2;miR-200c/141 fl/fl) and Ctrl (K8-CreERT2;miR-200c/141 fl/+) mice were FACS-isolated for RNA-seq analysis.

TISSUE(S): Mammary Gland

DISEASE(S): N/a

SUBMITTER: Ting,Wu

PROVIDER: OEX011154 | NODE |

REPOSITORIES: NODE

ACCESS DATA

Json Xml

Similar Datasets

Function of miR-200c/141 in mouse mammary gland

Project description:To investigate the changes of gene expression in mouse mammary gland after knockout of miR-200c/141 in mammary luminal cells. Luminal cells from CKO (K8-CreERT2;miR-200c/141 fl/fl) and Ctrl (K8-CreERT2;miR-200c/141 fl/+) mice were FACS-isolated for RNA-seq analysis.

| OEX011156 | NODE

Function of miR-200c/141 in mouse mammary gland

| OEX011155 | NODE

RNAseq of mouse mammary luminal cells

Project description:The mammary epithelia are mainly composed of two distinct lineages, the basal and luminal cells. In our MMTV-Cre; Brca1flox/flox mouse model, we found the Brca1 knockout mainly occurred in the luminal cells, which will lead the mammary tumorigenesis. To investigate the Brca1 deficiency mediated mammary tumorigenesis, we sorted the luminal cells from wild type mice and MMTV-Cre; Brca1flox/flox mice for RNAseq analysis.

2021-11-17 | GSE148613 | GEO

Insulin Receptor Loss Impairs Mammary Tumorigenesis in Mice

Project description:To investigate the role of Insr in processes affecting tumor initiation, we generated Comma1D luminal cells with a CRISPR Insr knockout. We then performed RNA-seq followed by differential gene expression analysis and pathway enrichment analysis.

2023-09-20 | GSE230583 | GEO

Expression data from mouse luminal mammary epithelial cells

Project description:We used a mouse strain in which one Tbx3 gene was replaced with the yellow fluorescent protein variant Venus. Luminal cells had either very high Tbx3 promoter activity or not at all. We performed an expression analysis on luminal mammary epithelial cells sorted based on their Venus expression (reporting Tbx3 promoter activity) to investigate the difference between these two cell populations. Mammary epithelial cells from 3 Tbx3-Venus-KI adult virgin female mice (FVB background) were pooled and luminal cells were sorted into a Venus-hi and a Venus-neg sample. There were no repeats for this study.

2014-08-20 | E-GEOD-58327 | biostudies-arrayexpress

Single cell RNA-seq reveals heterogeneity and hierarchy of mouse mammary epithelia

Project description:The mammary epithelia are mainly composed of two distinct lineages, the basal and luminal cells. Due to the limitation of traditional transcriptome analysis of bulk mammary cells, the hierarchy and heterogeneity of mammary cells within these two lineages remain unclear. Hence, we have performed single cell RNA-seq on mammary epithelial cells of virgin and pregnant mice. Based on the respective gene expression profiles, we found mammary cells are highly heterogeneous and can be classified into distinct subgroups with signature markers. Besides, we identified and verified a rare CDH5+ subpopulation of basal lineage as quiescent mammary gland stem cells (MaSCs). Furthermore, knockout of Cdh5 in mouse mammary cells via CRISPR-Cas9 significantly impairs cell stemness and promotes the cell differentiation to luminal lineage. In addition, via pseudo-temporal analysis, we reconstructed the mammary epithelia developmental trajectory and revealed the relevant changes of gene expression and biological functions of mammary cells along the developmental process.

2018-02-09 | GSE110371 | GEO

Expression data from mouse luminal mammary epithelial cells

2014-08-20 | GSE58327 | GEO

Microarray expression profiling of distinct subsets of mouse mammary epithelial cells

2014-11-26 | GSE47376 | GEO

Microarray expression profiling of distinct subsets of mouse mammary epithelial cells

Project description:The purpose of this microarray experiment was to obtain reference gene expression patterns of a number of epithelial cell populations [mammary stem cells (MASC), luminal progenitors (LP), alveolar luminal stem/progenitor cells (WC virgin-these are mammary epithelial cells genetically marked by Wap-Cre in virgin females), mature luminal cells (ML, mainly represent ductal luminal cells in virgin females), and alveolar luminal cells (WC preg M-bM-^@M-^S these are alveolar cells genetically marked by Wap-Cre during mid-gestation)] present in the mammary gland of wildtype adult mice on a C57BL6 genetic background. For the isolation of RNA from mammary stem cells (MASC, Lin-CD24+CD29hi), luminal progenitors (LP, Lin-CD24hiCD29+CD61+), and mature luminal cells (ML, lin-CD24hiCD29+CD61-), the thoracic and inguinal mammary glands from 3 adult virgin female mice were harvested, minced and digested into a single cell suspension. Form each of these 3 single cell suspensions, the above populations were sorted by FACS. For alveolar luminal stem/progenitor cells and alveolar luminal cells, Lin-YFP+ mammary epithelial cells were isolated from virgin or midgestation mice genetically marked by Wap-Cre;R26Y. R26Y is a conditional YFP reporter that would be turned on upon Cre-mediated recombination.

2014-11-26 | E-GEOD-47376 | biostudies-arrayexpress

Expression data from freshly isolated mouse mammary luminal cells

Project description:The mammary luminal cell compartment is heterogeneous. In adult virgin mice, it contains clonogenic luminal progenitors and non-clonogenic luminal cells expressing estrogen and progesterone receptors that can be discrimated by their cell surface expression of ICAM-1. We have separated ICAM1+ luminal progenitors and ICAM- non-clonogenic luminal cells and analyzed their transcriptomic profiles. After showing that the luminal progenitor population overexpressed Met, Trp53 and numerous p53 target genes, we analyzed how the p53 and Met signaling pathways cooperate to control the function and plasticity of luminal progenitors.

2019-02-05 | GSE122928 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data