Project description:Comparison of Arabidopsis T87 cells transformed with an empty vector vs transcription factor (TF)-overexpressed lines of T87 cells.

2007-03-15 | E-ATMX-8 | biostudies-arrayexpress

Changes in translational state from 1 day to 8 day after inoculation in Arabidopsis thaliana T87 cells

Project description:We investigated genome-wide changes in mRNA translation in Arabidopsis thaliana T87 suspension cell cultures which thought to be one of the host materials for bioreactor. Global translational repression was observed in cells of 8 day after inoculation that is thought to be stressful condition by the nutrient deficiency and hypoxia. This suggested the negative effect of the global translational repression on transgene expression. On the other hand, previous study using heat stress showed that some mRNAs were actively translated under such stressful condition, suggesting the existence of mRNA that were actively translated in cells of 8 day after inoculations. To identify mRNAs that escape global translational repression on 8 day and its cis-elements would be the 1st step to make the system for higher transgene expression by the escaping global translational repression. To this end, we subjected polysomal RNA and non-polysomal RNA from sucrose gradient fractionated cell lysates to the co-hybridization on Agilent Arabidopsis 4 Oligo Microarrays. The ratio of signal intensities (polysomal RNA: total RNA) was used as an indicator of the translation state for each transcript.

2013-12-10 | GSE52468 | GEO

Arabidopsis thaliana

Project description:TREseq analysis in Arabidopsis T87 cultured cell (pair-end sequencing)

| PRJDB10661 | ENA

Arabidopsis thaliana

Project description:Transcriptome and heterochromatin analysis of Arabidopsis thaliana T87 cultured cells

| PRJDB9046 | ENA

Arabidopsis thaliana

Project description:Transcription start site and epigenome analysis of Arabidopsis thaliana T87 cultured cells

| PRJDB9047 | ENA

Comparison of Arabidopsis callus

Project description:Arabidopsis thaliana plant expressing 35S:WIND1 shows callus-like morphology without hormone treatment. Transcriptomes of the callus-like cell expressing 35S:WIND1, callus of T87 cultured cell, 2,4-D-induced callus and control seedling plant were compared by Agilent microarray.

2011-05-18 | GSE21631 | GEO

Changes in translational state from 1 day to 8 day after inoculation in Arabidopsis thaliana T87 cells

2013-12-10 | E-GEOD-52468 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data