Project description:16S amplicon pool analyses of the four gut sections of the wood-feeding beetle, Odontotaenius disjunctus The beetle is purely wood feeding, and we aim to first characterize the community that exist within the gut sections
Project description:16S amplicon pool analyses of the four gut sections of the wood-feeding beetle, Odontotaenius disjunctus The beetle is purely wood feeding, and we aim to first characterize the community that exist within the gut sections 4 beetles, four gut sections per beetle, one PhyloChip per gut section, total = 16 chips
Project description:The pathogenic marine bacterium Vibrio vulnificus is a causative agent of food-borne diseases such as life-threatening septicemia and gastroenteritis in individuals with underlying predisposed conditions.Because most infection cases of V. vulnificus are caused by ingestion of contaminated seafood, the pathogen has to encounter host cells in the gut at the early stage of infection and alters its gene expression patters to adapt the gut environment of host. To identify differentially expressed genes when V. vulnificus cells are exposed to host cells, a transcriptome analysis of V. vulnificus cells that were either exposed to HT29-methotrexate intestinal epithelial cells or grown in basal medium eagle (Sigma) (as a negative control) was performed using Illumina GAIIx system. 16S and 23S rRNA were depleted using MICROBExpress kit (Ambion) from the total RNAs of each sample. Strand-specific cDNA libraries of the samples were constructed using Truseq stranded mRNA prep. kit (Illumina). Single-end and 36 cycle sequencing were performed using Iluumina GAIIx sequencer by ChunLab (http://www.chunlab.com) and Raw sequence data were analyzed by CLC Genomics Workbench 5. 5. 1 (CLC Bio)
Project description:A phylogenetic microarray targeting 66 families described in the human gut microbiota has been developped aud used to monitor the gut microbiota's structure and diversity. The microarray format provided by Agilent and used in this study is 8x15K. A study with a total of 4 chips was realized. Arrays 1 and 2: Hybridization with 100ng of labelled 16S rRNA gene amplicons from a mock community sample and 250ng of labelled 16S rRNA gene amplicons from 1 faecal sample. Each Agilent-030618 array probe (4441) was synthetized in three replicates. Arrays 3 and 4: Hybridization with 250ng of labelled 16S rRNA gene amplicons from 2 faecal samples. Each Agilent-40558 array probe (4441) was synthetized in three replicates.
Project description:Given the criticle role of gut bacteria involve in number of diseases, the gut microbiota from young and aged people were estimated using 16s rRNA next-generation sequencing. This study will benefit to identify the role of gut bacteria on the pathegenic mechasim of aging relative diseases.
Project description:Gut microbial profiling of uterine fibroids (UFs) patients comparing control subjects. The gut microbiota was examined by 16S rRNA quantitative arrays and bioinformatics analysis. The goal was to reveal alterations in the gut microbiome of uterine fibroids patients.
Project description:To explore the effects of gut microbiota of young (8 weeks) or old mice (18～20 months) on stroke, feces of young (Y1-Y9) and old mice (O6-O16) were collected and analyzed by 16s rRNA sequencing. Then stroke model was established on young mouse receive feces from old mouse (DOT1-15) and young mouse receive feces from young mouse (DYT1-15). 16s rRNA sequencing were also performed for those young mice received feces from young and old mice.
Project description:Gut microbiota were assessed in 540 colonoscopy-screened adults by 16S rRNA gene sequencing of stool samples. Investigators compared gut microbiota diversity, overall composition, and normalized taxon abundance among these groups.
