Project description:Schistosoma japonicum causes severe disease or even death and liver fibrosis is the major damage, which is caused by immunopathological injury. However, a comprehensive understanding of the immune cell changes during S. japonicum infection is lacking. Here, we performed single-cell RNA-sequencing and T/B cell receptor sequencing (scRNA-seq/scTCR-seq/scBCR-seq) of the total mouse splenocytes after cercaria infection.The changes of immune cells with advancing disease stages were analyzed and the relationship with liver fibrosis was investigated.
Project description:We compared the transcriptomes of Amphioxus (Branchiostoma lanceolatum) control and SU5402 treated embryos to define putative FGF signalling pathway target genes during somitogenesis. Embryos were treated with 50µM of SU5402 from 5.5hpf to 8.5hpf, 11.5hpf, and 14.5hpf or from 15hpf to 18hpf, 21hpf, 24hpf. Total RNA was extracted from control and treated embryos.
