Project description:Ovarian cancer is one of the most lethal gynaecological malignancies among female population. The high mortality rate of this disease is due to its poor prognosis and most patients present at an advanced stage accompanied with metastasis. The frequent tumor recurrence rate associated with metastasis and chemoresistance are the major obstacles in clinical management of the disease. Different from other solid tumors, ovarian cancer perfers transcoelomic metastasis amd mounting evidence has suggested ovarian cancer patients who have peritoneal metastasis are usually poor prognosis and highly chemoresistant recurrence. In this study, we examined the differential gene expression profile between the primary tumors and their paired metastastic lesions with a view to discover novel target therapies in combating ovarian cancer.
Project description:LSD1 is frequently overexpressed and correlates with poor prognosis in patients with ovarian cancer. RNA-seq, CUT&Tag-seq and ATAC-seq were performed to determine the impact of genetic depletion or pharmacological inhibition of LSD1 on gene expresion profiles in ovarian cancer cells.
