Aspergillus oryzae RIB40
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REPOSITORIES: ENA
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Project description:The Aspergillus oryzae, an important filamentous fungus used in food fermentation and enzyme industry, has been revealed to own prominent features in its genomic compositions by genome sequencing and various other tools. However, the functional complexity of the A. oryzae transcriptome has not yet been fully elucidated. Here, we applied direct high-throughput paired-end RNA sequencing (RNA-Seq) to the transcriptome of A. oryzae under four different culture conditions and confirmed most of the annotated genes. Moreover, with high resolution and sensitivity afforded by RNA-Seq, we were able to identify a substantial number of novel transcripts, new exons, untranslated regions, alternative upstream initiation codons (uATGs) and upstream open reading frames (uORFs), which serves a remarkable insight into the A. oryzae transcriptome. We also were able to assess the alternative mRNA isoforms in A. oryzae and found a large number of genes undergoing alternative splicing. Many genes or pathways that might involve in higher levels of protein production in solid-state culture than in liquid culture were identified by comparing gene expression levels between different cultures. Our analysis indicated that the transcriptome of A. oryzae was much more complex than previously anticipated and the results might provide a blueprint for further study of A. oryzae transcriptome. mRNA expression of Aspergillus oryzae in 4 different culture conditions was determined by method of RNA-Seq using short reads from high throughput sequencing technology.
2010-02-02 | E-GEOD-18851 | biostudies-arrayexpress
Project description:Aspergillus oryzae RIB40 Genome sequencing and assembly
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Project description:Expression data from Aspergillus oryzae grown under hypoxic condition.
| PRJNA144865 | ENA
Project description:Top 200 scaffolds from de novo assembly of Aspergillus oryzae RIB326 genome using short reads
| PRJDB45 | ENA
Project description:Expression analysis of Aspergillus oryzae grown on different carbon sources
| PRJNA212019 | ENA
Project description:The Aspergillus oryzae, an important filamentous fungus used in food fermentation and enzyme industry, has been revealed to own prominent features in its genomic compositions by genome sequencing and various other tools. However, the functional complexity of the A. oryzae transcriptome has not yet been fully elucidated. Here, we applied direct high-throughput paired-end RNA sequencing (RNA-Seq) to the transcriptome of A. oryzae under four different culture conditions and confirmed most of the annotated genes. Moreover, with high resolution and sensitivity afforded by RNA-Seq, we were able to identify a substantial number of novel transcripts, new exons, untranslated regions, alternative upstream initiation codons (uATGs) and upstream open reading frames (uORFs), which serves a remarkable insight into the A. oryzae transcriptome. We also were able to assess the alternative mRNA isoforms in A. oryzae and found a large number of genes undergoing alternative splicing. Many genes or pathways that might involve in higher levels of protein production in solid-state culture than in liquid culture were identified by comparing gene expression levels between different cultures. Our analysis indicated that the transcriptome of A. oryzae was much more complex than previously anticipated and the results might provide a blueprint for further study of A. oryzae transcriptome.
2010-02-02 | GSE18851 | GEO
Project description:Mycelial penetration in rice koji and nuclear increase of Aspergillus oryzae
| PRJDB9259 | ENA
Project description:Effects of manR disruption and overexpression on Aspergillus oryzae gene expression
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Project description:Aspergillus oryzae gene expression with 1hr galactose, glucose, and sorbitol induction.
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Project description:We aimed to elucidate biological function of A Disintegrin and Metalloprotease (Adm) A and AdmB in Aspergillus oryzae. To observe influences of admA and admB deletions, each gene deletion strain was analyzed by RNA-Seq. mRNA of Deletion-control, ∆admA∆admB, ∆admA and ∆admB cultivated in wheat bran solid culture were sequenced, in quintuplicate, using Illumina GAIIx.
2019-06-07 | GSE83207 | GEO