Project description:Transcriptional profiling of sugarcane leaf (+1) was collected from drought-prone areas at 42 and 117 days after the last rainfall when plants were 6- and 9-month-old, respectively. Sample hybridization (non-irrigated treatment against irrigated control) was performed to monitor gene expression in a two-color Agilent custom microarray. Our goal was to investigate the effect of mild and severe water stress on the transcriptomes of drought-sensitive (IACSP97-7065) and -tolerant (IACSP94-2094) sugarcane genotypes.
Project description:We tested whether home field advantage at inter- and intra-specific levels alters microbial carbon transformations, using a multi-factorial design with microcosms of freshwater submerged leaf litter from two species (Alder and Hemlock) exposed to 'home' or 'away' communities of microbes isolated from decomposing leaf litter, as well as controls with no microbial community added. For all 'home', 'away' and control conditions for each species we also had high or low oxygen treatments. Samples were taken at day 0, 154, 257 and 354 and extracted with solid phase columns and methanol to provide extracted metabolites.
